COVID-19冠状病毒自身无疑是其相关研究的关键试剂,但是获得可观的病毒剂量并非易事。面这对些问题,最佳解决方式是采用反向遗传学的方法,即利用该病毒的人工重组载体来重构病毒本身。具体方法是,构建携带目的冠状病毒基因组的DNA载体,然后将其转染到包装细胞中来产生大量活病毒。该方法的优势在于,可以便捷地进行研究所需要的载体修饰,如添加报告基因(如EGFP)或引入特定的突变。此外,鉴于野生型冠状病毒的基因组为正义单链RNA,而该种方式制备重组冠状病毒的病毒基因组模板为DNA,载体本身并不具有传染性。冠状病毒载体可以安全地使用并以最低程度的生物安全规范进行运输。
我们提供的的冠状病毒载体系统适用于生产重组活病毒。用户只需将我们的载体转染到合适的包装细胞中,即可重构任何种类的活性冠状病毒。用户得到的病毒可以在适当的细胞系中进一步扩增以达到研究所需要的剂量。
赛业生物科技与云舟生物科技合作,为您的冠状病毒研究提供冠状病毒载体构建服务。您可以在以下链接咨询我们的冠状病毒载体构建服务:点击此处发送载体设计咨询。
关于冠状病毒的更多细节信息如下表所示:
病毒代号 | 病毒全名 | GenBank信息 | 所属家族 | 宿主 | 宿主受体 | 引发疾病 | 刺突蛋白(S蛋白)序列 |
---|---|---|---|---|---|---|---|
HCoV-229E | Human coronavirus 229E | NC_002645.1 | Alphacoronavirus | Human (Homo sapiens) | ANPEP; Cell entry aided by TMPRSS2 | Common cold | View Sequence |
HCoV-NL63 | Human Coronavirus NL63 | NC_005831.2 | Alphacoronavirus | Human (Homo sapiens) | ACE2; Cell entry aided by TMPRSS2 | Common cold | View Sequence |
PEDV | Porcine epidemic diarrhea virus | NC_003436.1 | Alphacoronavirus | Pig (Sus scrofa) | ANPEP | Porcine epidemic diarrhea | View Sequence |
PRCoV | Porcine respiratory coronavirus | DQ811787.1 | Alphacoronavirus | Pig (Sus scrofa) | ANPEP | Porcine respiratory disease complex | View Sequence |
TGEV | Transmissible gastroenteritis virus strain PUR46-MAD | NC_038861.1 | Alphacoronavirus | Pig (Sus scrofa) | ANPEP | Transmissible gastroenteritis | View Sequence |
CCoV | Canine enteric coronavirus serotype II strain 1-71 | JQ404409.1 | Alphacoronavirus | Dog (Canis lupus familiaris) | ANPEP | Gastroenteritis | View Sequence |
FCoV | Feline coronavirus serotype I strain Black | EU186072.1 | Alphacoronavirus | Cat (Felis catus) | ANPEP | Feline infectious peritonitis | View Sequence |
FIPV | Feline infectious peritonitis virus serotype II | NC_002306.3 | Alphacoronavirus | Cat (Felis catus) | ANPEP | Feline infectious peritonitis | View Sequence |
SARS-CoV-2 | SARS-CoV-2 strain H1 | NC_045512.2 | Betacoronavirus | Human (Homo sapiens) | ACE2; Cell entry aided by TMPRSS2 | COVID-19 | View Sequence |
BatCoV-RaTG13 | Bat COVID-19-related coronavirus RaTG13 | MN996532.1 | Betacoronavirus | Chinese horseshoe bat (Rhinolophus sinicus) | View Sequence | ||
SARS-CoV | SARS-CoV strain Tor2 | NC_004718.3 | Betacoronavirus | Human (Homo sapiens) | ACE2; Cell entry aided by TMPRSS2 | SARS | View Sequence |
BatCoV-WIV16 | Bat SARS-related coronavirus WIV16 | KT444582.1 | Betacoronavirus | Chinese horseshoe bat (Rhinolophus sinicus) | ACE2 | View Sequence | |
BatCoV-Rs4231 | Bat SARS-related coronavirus Rs4231 | KY417146.1 | Betacoronavirus | Chinese horseshoe bat (Rhinolophus sinicus) | ACE2 | View Sequence | |
BatCoV-RsSHC014 | Bat SARS-related coronavirus RsSHC014 | KC881005.1 | Betacoronavirus | Chinese horseshoe bat (Rhinolophus sinicus) | ACE2 | View Sequence | |
BatCoV-WIV1 | Bat SARS-related coronavirus WIV1 | KF367457.1 | Betacoronavirus | Chinese horseshoe bat (Rhinolophus sinicus) | ACE2 | View Sequence | |
HCoV-OC43 | Human coronavirus OC43 | NC_006213.1 | Betacoronavirus | Human (Homo sapiens) | Sialic acid; Cell entry aided by TMPRSS2 | Common cold | View Sequence |
CRCoV | Canine respiratory coronavirus | JX860640.1 | Betacoronavirus | Dog (Canis lupus familiaris) | Canine respiratory disease | View Sequence | |
BCoV | Bovine Coronavirus | NC_003045.1 | Betacoronavirus | Cow (Bos taurus) | Sialic acid | Calf diarrhea and respiratory infections in all ages | View Sequence |
PHEV | Porcine hemagglutinating encephalomyelitis virus | DQ011855.1 | Betacoronavirus | Pig (Sus scrofa) | Porcine hemagglutinating encephalomyelitis | View Sequence | |
ECoV | Equine Coronavirus | EF446615.1 | Betacoronavirus | Horse (Equus caballus) | Colic | View Sequence | |
HCoV-HKU1 | Human coronavirus HKU1 | NC_006577.2 | Betacoronavirus | Human (Homo sapiens) | Sialic acid; Cell entry aided by TMPRSS2 | Common cold | View Sequence |
MHV | Mouse hepatitis virus strain A59 | AY700211.1 | Betacoronavirus | Mouse (Mus musculus) | Ceacam1 | Mouse hepatitis | View Sequence |
RCoV | Rat CoV strain Parker | NC_012936.1 | Betacoronavirus | Rat (Rattus norvegicus) | Pneumonitis | View Sequence | |
MERS-CoV | MERS-CoV | NC_019843.3 | Betacoronavirus | Human (Homo sapiens) | DPP4; Cell entry aided by TMPRSS2 | MERS | View Sequence |
BatCoV-HKU4 | Bat MERS-related Coronavirus HKU4 | NC_009019.1 | Betacoronavirus | Lesser bamboo bat (Tylonycteris pachypus) | View Sequence | ||
BatCoV-HKU5 | Bat MERS-related coronavirus HKU5 | NC_009020.1 | Betacoronavirus | Japanese house bat (Pipistrellus abramus) | View Sequence | ||
IBV | Infectious bronchitis virus | NC_001451.1 | Gammacoronavirus | Chicken (Gallus gallus) | Sialic acid | Avian infectious bronchitis | View Sequence |
TCoV | Turkey coronavirus | NC_010800.1 | Gammacoronavirus | Turkey (Meleagris gallopavo) | Type 2 poly-N-acetyl-lactosamines | Gastroenteritis | View Sequence |
PDCoV | Porcine deltacoronavirus | KJ584355.1 | Deltacoronavirus | Pig (Sus scrofa) | ANPEP | Diarrhea | View Sequence |