Knockout Cell Line Only $5,599

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  Efficient Smart-CRISPR™ Technology

  Allows for multiple knock-out strategies, higher gene editing efficiency, and ensures non-expression of functional protein.
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  Superior Customer Support

  VIP project specialist is available for project follow-up and progress reporting support.
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  Extensive Experience

  Over 1,000 cell models successfully generated, receiving over 100 peer-reviewed citations thus far.
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  Quality Control

  Thorough validation and testing are performed to ensure that the cell line model meets your requirements.
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  One-stop Solution

  Cyagen offers comprehensive solutions for both in vivo and in vitro studies, from gene expression regulation and gene function verification across various cells, to establishment of mouse or rat models, as well as downstream phenotypic analysis.

Project: Human HCT116 cell PSME3 gene knockout (fragment deletion)

1. gRNA Design gRNA Site 1: ATACGCTACCTAACATGGCTGGG； gRNA Site 2: CCAGCATGCAAAATACAATGGGG	2. Primer Synthesis and Plasmid Construction
3. Cell Transfection Firstly, confirm the optimal electroporation parameters, then transfect the vector into cells by electroporation.	4. Single Cell Clone Screening and Culture
5. PCR and Electrophoresis Identification Identification Strategy:	6. Sequencing Identification ATAAACAGAGGATTTAAGACTTTTGTTATGTTTTAGACGC-del； 1809bp--AGTGGCTAATGCCT GTAATCC CACCACTTTGGGAGGCCAA

1. gRNA Design gRNA Site 1: ATACGCTACCTAACATGGCTGGG； gRNA Site 2: CCAGCATGCAAAATACAATGGGG

2. Primer Synthesis and Plasmid Construction

3. Cell Transfection Firstly, confirm the optimal electroporation parameters, then transfect the vector into cells by electroporation.

4. Single Cell Clone Screening and Culture

5. PCR and Electrophoresis Identification Identification Strategy:

6. Sequencing Identification ATAAACAGAGGATTTAAGACTTTTGTTATGTTTTAGACGC-del； 1809bp--AGTGGCTAATGCCT GTAATCC CACCACTTTGGGAGGCCAA

Featured Cell Lines （Successfully Generated）