Price Match Custom Models

Are you considering custom animal models for your next research project? Do you want to maximize your research budget? Interested in securing the added confidence of a guaranteed genotype*?

Lock-in the best deal with our limited-time “Price Match + 10% Off” custom animal model (CAM) project promotion! Get the most cost-effective answer to all of your CAM project requirements by allowing us to match any quote from commercial providers (for equivalent services) with an additional 10% off.

As a one-stop shop for research model solutions, Cyagen offers complete outsourcing for all your animal model needs through The Alliance—a unique partnership that provides specially tailored model generation and colony management solutions for the academic and non-profit markets. Extend your research budget with cost-effective and reliable CAM development projects and supporting services.

● Promotion Period: Offer ends December 31st, 2022.

● Eligibility: Academic end clients in Europe, North America, and South America.

Our custom animal model services offer the following advantages:

Price Matching + 10% Off (equivalent scope of work)

Our team will price match CAM project quotes from commercial providers and give you an extra 10% discount to help ensure researchers get the best deal for their study. Bring your quote, and we will match it.

Guaranteed Project Genotype

Cyagen’s gene editing services are unparalleled in delivering research-ready rodent models with a guaranteed genotype. To optimize the process of generating your new model, Cyagen provides a unique genotype guarantee* - confirmed using standard PCR validation of the expected fragment size to match the original project strategy - to give you further peace of mind.

*Does not guarantee biological outcome (i.e., transgene expression levels) and is limited by modifications that may negatively affect the health and viability.

Fast Project Scoping & Quotes

We will work with you to identify the optimal CAM project strategy to serve your research goals and suit your budget.

Comprehensive Capabilities

Provides researchers with access to a comprehensive toolkit of technologies, including but not limited to: CRISPR/Cas9, transgenic technology, PiggyBac, and embryonic stem cell (ESC)-based homologous recombination (HR).

Custom Model Capabilities >>

The key to fast, reliable custom model generation for academic and non-profit research projects is the Taconic-Cyagen Academic Model Generation Alliance (The Alliance). The Alliance provides specially tailored model generation and colony management solutions for the academic and non-profit markets—ensuring you can obtain reliable animal models that suit your budgets and timelines, while reducing import quarantine requirements and delays.

Taconic Biosciences is the leader in custom model generation and distribution, with 60+ years of experience providing relevant mouse and rat models to investigators worldwide. When paired with Cyagen’s highly efficient processes, our partnership redefines what academic and non-profit researchers can expect from an animal model partner, providing a turnkey, streamlined experience from model design to generation to importation. We even reduce the barriers to obtaining the right model for your study through fully licensed technologies, competitive pricing, and delivery options that allow for direct import into your facility to delay.

As partners, Taconic and Cyagen leverage the power of our complementary approaches to provide you with the best, most competitive solutions for your projects.

Comprehensive, Flexible CAM Capabilities

The Alliance integrates all the capabilities required for a successful academic or non-profit research project.

● Fast project scoping: We use AI-powered tools to generate project quotes in as little as two days.
● Accelerated timelines: We can rapidly generate age-matched cohorts of 20-40 animals at any Taconic health standard, significantly reducing traditional breeding timelines.
● Cost-effective, guaranteed model generation: Our guaranteed deliverables facilitate your discovery work, and our focus on value maximizes your research budget.
● Comprehensive model generation toolkit: We develop your custom model using the most appropriate technology for your study objectives, with options that include CRISPR/Cas9, transgenic technology, PiggyBac, and embryonic stem cell (ESC)-based homologous recombination (HR).
● Flexible delivery: No matter what your vivarium’s requirements, we can deliver your custom model to your facility without the delays and costs of quarantine through our TruIMPORT™ services.

● Wide Range of Model Types

■ Knockouts (KO), Conditional Knockouts and Knock-ins (cKO/cKI)
■ Point Mutation Knock-ins, Large-fragment Knock-ins (LFKI)
■ Transgenics
■ Humanization

● Multiple Donor Strains Available

■ Mouse: C57BL/6, FVB, BALB/c
■ Rat: Sprague Dawley® (SD), Wistar, F344, Long Evans, Brown Norway

TruIMPORT: Flexible Delivery and Importation Options

The Alliance integrates all the capabilities required for a successful academic or non-profit research project.

Our unique RapidRELAY™, RapidCHECK™, and RapidEXPANSION™ services eliminate common barriers to importing a new model into your facility. You can work with your facility veterinarian to choose the option that best meets your vivarium’s requirements, enabling you to reduce the time and added costs of animal.

Supporting Services: downstream (homozygous) breeding, cryopreservation, and more

Mouse and Rat Breeding

Cyagen’s breeding service provides reliable, economical, and efficient breeding colonies of mice/rats to support your research needs, allowing you to focus more on your research goals and less on animal care, breeding, and colony management.
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Cryopreservation

Cyagen can back up your live animal colony through our embryo and sperm cryopreservation services, which can not only protect your valuable strains but also reduce your cost in the long run. We also can produce live animals for you in eight to twelve weeks once your cryopreserved stock is preserved at Cyagen.
Learn More >>
Phenotype Analysis Services

Our phenotyping services include surgical model construction alongside analytics capabilities for blood, tissue histology and morphology, pathology, genomic and proteomic expression, behavioral and metabolic analysis, and more.
Learn More >>
Please contact us if you need additional information.

Technology: TurboKnockout^®, CRISPR, Transgenic, PiggyBac, ESC-based HR, and more

TurboKnockout^® - Proprietary Method for Accelerated Generation of Precise Mouse Models
Cyagen’s Proprietary Method for Accelerated Generation of Precise Mouse Models

Our proprietary TurboKnockout^® gene targeting method can provide you with conditional knockout, reporter knockin and humanization mouse models as fast as 6-8 months. This proprietary gene targeting method is enabled by two innovations:
- ● Eliminates the unpredictability of germline transmission (GLT)with our super competent ESC line that generates 100% ESC-derived founder mice rather than chimeras.
- ● Self-removing selection cassette circumvents the need to breed to Flp deleter mice
These innovations eliminate at least two generations of breeding, shortening production time by 4-6 months as compared to the industry standard

Highlights of TurboKnockout^® Gene Targeting

TurboKnockout^® gene targeting: 6-8 months
- Targeting Vector
  Construction
  2 months
- Gene Targeting
  in ES Cells
  2-3 months
- Founder Het
  Generation
  2-3 months
Traditional gene targeting: 10-14 months
- Targeting Vector
  Construction
  2 months
- Gene Targeting
  in ES Cells
  2-3 months
- Chimera
  Generation
  2-3 months
- F1 Het
  Generation
  2-3 months
- Removal of
  Selection Cassette
  2-3 months
Highlights of TurboKnockout^® Gene Targeting
- ● No patent risk: Technology of choice for drug development projects
- ● Success rate: 100% guaranteed germline transmission (GLT)
- ● Turnaround time: Founders as fast as 6-8 months
- ● Large fragment knockin size: Knockin (KI) fragment size up to 200 kb
- ● Limitless loxP: No size limitations for your floxed region
- ● 100% Money-Back Guarantee on TurboKnockout^® projects
Capabilities

Donor Strains Mouse: C57BL/6, BALB/c
Cyagen CRISPR-Pro Technology - Capabilities for Mouse & Rat Models
Engineered nuclease-mediated genome editing (especially CRISPR/Cas9) is an emerging technology which can serve as an alternative to the conventional, ES cell homologous recombination-based knockout (KO) animal generation. These methods have enabled the generation of custom mouse and rat models with genetic knockouts, point mutations, and large fragment knockins (LFKIs).

Cyagen’s CRISPR-based Rapid Knockout services use accelerated methodology that provides conventional knockout, reporter knockin, and humanization founder animals in as fast as 3 months.

Capabilities

Donor Strain Information:
- ● Mouse strains: C57BL/6, FVB
- ● Rat strains: Sprague Dawley (SD), Long Evans
- Note: Other strains available upon request.

Transgenic Capabilities for Animal Model Generation

Items	Regular Transgenic	PiggyBac Transgenic	Rosa26 Targeted Transgenic
Integration	Random, multicopy integration	Random, single copy per integration site	Single copy transgene targeted to Rosa26 safe harbor locus
Vector construction	Plasmid or BAC	Plasmid or BAC	CRISPR or TurboKnockout^®
Expression pattern	Variable	More consistent	Most consistent
Endogenous effects	Can disrupt endogenous genes	Less likely to disrupt endogenous genes	Safe harbor locus
Donor Strain Information	Mouse strains: C57BL/6, FVB Rat strains: Sprague-Dawley (SD), Long Evans Note: Other strains available upon request.

PiggyBac transgenic services can deliver single-copy transgenic animals with more consistent expression of your transgene. This proprietary transgenic method has the following advantages over other transgenic approaches:

◆ Single-copy integration: Avoids potential gene silencing from multiple copies
◆ Defined region of integration: No loss of transgene sequence (TTAA, transcription unit)
◆ Reliability: More consistent expression pattern compared to plasmid based transgenics
◆ Economical: Cost and turnaround time comparable to plasmid based transgenics

Feature Citation on Custom Rodent Models

Research Applications	Gene Target(s)	Journal Information
Immunology, Gut	Ddx58	Commensal viruses maintain intestinal intraepithelial lymphocytes via noncanonical RIG-I signaling. Nature Immunology PMID: 31636462
Cell Biology, Cardiovascular	SAHH(Ahcy)	Inhibition of S-Adenosyl homocysteine Hydrolase Induces Endothelial Dysfunction via Epigenetic Regulation of p66shc-Mediated Oxidative Stress Pathway Circulation DOI: 10.1161/circulationaha.118.036336
Oncology	Trib3	TRIB3 Promotes APL Progression through Stabilization of the Oncoprotein PML-RARa and Inhibition of p53-Mediated Senescence Cancer Cell DOI: 10.1016/j.ccell.2017.04.006

Case Studies
Testimonials

>> Case Study: Intestinal Epithelial Cells Regulate the Differentiation of Intraepithelial Lymphocytes through the Expression of MHC II and PD-L1

>> Case Study: LARP7-Mediated U6 snRNA Modification and Its Role in Pre-mRNA Splicing As Well As Its Functions in Mouse Spermatogenesis

>> Case Study: Novel Mouse Model for Researching RAB7A and NF-kB Activation

"We generated a mouse knockout model using Cyagen’s TurboKnockout^® technology. Cyagen’s team was on time with milestones, provided constant updates on the project and delivered animals as promised. It was such a pleasure working with Cyagen that we decided to outsource the production of other animal models to them."

———— Maximiliano D’Angelo, Sanford Burnham Prebys Medical Discovery Institute

"Using Cyagen’s CRISPR-generated constitutive knockout mice has greatly facilitated our understanding the biology in IL-1 family of 11 cytokines or their respective receptors. We were able to have the IL-38 deficient mice and the IL-1R9 deficient mice in record time. The service provided by Cyagen is highly recommended."

———— Charles A. Dinarello, University of Colorado Denver - Anschutz Medical Campus

"Our lab has been using Cyagen’s transient transgenic mouse embryo LacZ service to test candidate sequences for enhancer activity for over five years (over 60 different sequences tested with them thus far). Their cost, turnaround time, submission process and customer service has been great, making this assay easy and routine for our lab."

———— Nadav Ahituv, University of California, San Francisco

Discover more >>

Price Match + 10% Off Custom Models