B6J-Apoe KO Mice

The target sequence of this strain was designed to include exons 2-4 of the mouse Apoe gene.

● Cardiovascular disease research: Hypercholesterolemia, hyperlipidemia, atherosclerosis, etc.;

● Body metabolism mechanism research: Fat and cholesterol metabolism;

● Neurodegenerative disease research: Alzheimer's disease.

1. Expression of the APOE protein

Figure 1. Expression of APOE protein in the livers of 8-week-old male B6J-Apoe KO and wild-type (WT) mice. Western blot results showed no expression of APOE protein in the livers of B6J-Apoe KO mice.

2. Growth Curves

Figure 2. Body weight trajectories of B6J-Apoe KO and wild-type (WT) mice on normal diet (ND) and high-fat diet (HFD) conditions. Results showed that B6J-Apoe KO mice grew at a similar rate in normal and HFD conditions. WT mice fed HFD gained weight, and internal inspection data showed that at 20 weeks, the aortic arch and aortic vessels in WT mice were normal and no atherosclerotic plaque was formed.*
*High-fat diet was purchased from Medicience (catalog number: MD12015). High-fat feeding protocol: Mice were started on a gradual transition to the high-fat diet at week 5 and began formal feeding on the high-fat diet at week 6.

3. Cholesterol blood test in male mice

Figure 3. Serum lipid biochemical indicators of male B6J-Apoe KO and wild-type (WT) mice on normal diet and high-fat diet (HFD) conditions. Data showed that under normal diet conditions, compared with wild-type mice, male B6J-Apoe KO mice had different degrees of increases in triglyceride (TRIG), cholesterol (CHOL), and low-density lipoprotein (LDL-C) levels in their bodies. High-fat diet further increased the levels of these indicators in male B6J-Apoe KO mice. The levels of high-density lipoprotein (HDL-C) in male B6J-Apoe KO mice and wild-type (WT) mice were not significantly different.

4. Cholesterol blood test in female mice

Figure 4. Serum lipid biochemical indicators of female B6J-Apoe KO and wild-type (WT) mice on normal diet and high-fat diet (HFD) conditions. Under normal diet conditions, compared with wild-type mice, female B6J-Apoe KO mice had significantly elevated levels of cholesterol (CHOL) and low-density lipoprotein (LDL-C). High-fat diet further increased the levels of cholesterol (CHOL) and low-density lipoprotein (LDL-C) in female B6J-Apoe KO mice. The levels of high-density lipoprotein (HDL-C) and triglyceride (TRIG) in female B6J-Apoe KO mice and wild-type (WT) mice were not significantly different.

5. Aortic pathology in male B6J-Apoe KO mice

Figure 5. Detection of atherosclerotic plaques in the aorta of male B6J-Apoe KO mice on normal diet (ND) and high-fat diet (HFD) conditions. Male B6J-Apoe KO mice can spontaneously form atherosclerotic plaques in the aorta after 10 weeks on a normal diet, with mild symptoms. After induction by high-fat diet feeding, the atherosclerotic plaques in the aorta were exacerbated, and the symptoms became more obvious after week 12, continued to worsen at week 14, and reached the late stage of pathological phenotype at week 16.

6. Aortic pathology in female B6J-Apoe KO mice

Figure 6. Detection of atherosclerotic plaques in the aorta of female B6J-Apoe KO mice on normal diet (ND) and high-fat diet (HFD) conditions. Female B6J-Apoe KO mice can spontaneously form atherosclerotic plaques in the aorta after 12 weeks on a normal diet, with mild symptoms. After induction by high-fat diet feeding, the atherosclerotic plaques in the aorta were exacerbated, and the symptoms became more obvious after week 14 and continued to worsen at week 16.

• 7. Oil Red O staining of the aorta
  
  
  

  
  Figure 7. Oil Red O staining results for B6J-Apoe KO mice and wild-type mice (WT) under chow diet (CD) and high-fat diet (HFD) conditions. The red color indicates positive fat staining. The results show that wild-type mice, after 20 weeks of both chow and high-fat diets, exhibited no significant arterial lesions. However, B6J-Apoe KO mice, following a high-fat diet, showed noticeable fat accumulation near the aortic arch, with a more severe phenotype after 20 weeks. Similarly, B6J-Apoe KO mice, after a chow diet, also exhibited spontaneous atherosclerosis, which worsened after 20 weeks.

8. Oil Red O staining of cardiac valves

Figure 8. Oil Red O staining of cardiac valves for B6J-Apoe KO mice and wild-type mice (WT) under chow diet (CD) and high-fat diet (HFD) conditions. The results show that wild-type mice, after 20 weeks of both chow and high-fat diets, exhibited no fat accumulation near the cardiac valves. However, B6J-Apoe KO mice, following a high-fat diet, showed noticeable fat accumulation near the cardiac valves, with a more severe phenotype after 20 weeks. Similarly, B6J-Apoe KO mice also exhibited fat accumulation under a chow diet, which worsened after 20 weeks.

• Temperature: 20-26°C
• Humidity: 40%-70%
• Bedding: Change high-pressure sterilized bedding weekly.
• Normal Chow Diet: Standard Maintenance Diet (Jiangsu Xietong Pharmaceutical Bio-engineering, catalog number: SWC9101).
• High-fat Diet: HFD Diet (Medicience, catalog number: MD12015).
• Suggested Modeling Period: Begin high-fat feeding from 5-8 weeks of age. After 1 week of adaptive feeding with a high-fat diet (as shown in Table 1), switch to a complete high-fat diet. Tissue collection after 12-16 weeks of high-fat feeding may reveal atherosclerotic lesions in the aorta.

Table 1: Adaptive feeding conditions for a high-fat diet

Day	Condition
Day 1-3	Standard: High-fat diet ratio = 2:1
Day 4-5	Standard: High-fat diet ratio = 1:1
Day 6-7	Standard: High-fat diet ratio = 1:2

 

*Dietary effects on this model may vary based on environmental and feeding conditions. Data provided is for reference to help you optimize study design.