● What is a Knockout Cell Line?
● Applications of Knockout Cell Line Models
● How to Effectively Generate CRISPR Gene Knockout Cell Lines
● How to Choose a Suitable Transfection Method
● How to Avoid the Risk of Residual Protein Expression
Cyagen Exclusive White Paper:
Application of CRISPR/Cas-based Technology to Create Knockout Cell Lines
Generation of knockout (KO) cell line models with a loss-of-function mutation is an important method for studying the function of given genes. Compared with traditional gene interference (RNAi) technology, gene knockout (KO) models allow researchers to fully disrupt expression of the target gene, ultimately leading to either complete non-expression or loss of function in proteins. Gene knockout (KO) cell lines enable researchers to study the role of specific genes by comparing the phenotypic differences among the knockout and wildtype cells.
In this White Paper, we review the use of CRISPR/Cas-based technology to create knockout (KO) cell lines and provides practical strategies for you to effectively generate a KO cell line model.
Download this Exclusive White Paper to discover how to create optimal KO cell lines for your research>>
Outline of Content
Cyagen’s Knockout Cell Line Services
Cyagen’s Smart-CRISPR™ cell line modeling services enable large fragment knockout and/or accurate mutation(s) – providing knockout cell lines with complete loss-of-function of the target gene.
Our custom cell line modeling services platform features CRISPR-mediated gene editing that is optimally targeted with our proprietary artificial intelligence (AI)-based AlphaKnockout Smart Gene Targeting System - enabling higher knockout (KO) efficiency. We can perform multiple knockout strategies, including frameshift mutation, large fragment knockout, and multiple genes knockout. With CRISPR/Cas9 technology, researchers can easily solve the problem of residual protein expression seen in RNA interference (RNAi) gene knockdown models.
