Overview

The Cyagen Transgenic Animal Center (CTAC) is a specific pathogen-free (SPF) barrier facility located in China that houses 150,000 cages of mice. The facility's housing space is divided into separate barrier areas, each with independent restricted access, ventilation systems, and animal care staff.

Accreditations

CTAC has been accredited and certified by various organizations on different aspects, including ISO 9001 and AAALAC Full Accreditation. All these accreditation ensure that our services and products meet internationally recognized quality standards.

Regulatory Inspections

CTAC follows the "Regulations on the Administration of Laboratory Animals" promulgated by the State Council of the People's Republic of China and is inspected by the Chinese government every two years. The AAALAC inspection is performed every three years in order to maintain accredited status.

Facility Security

CTAC is located in a fenced-off business park with 24/7 security personnel. The facility is fully monitored by multiple CCTV cameras and security/maintenance personnel are on duty at all times. A backup power generator is available in the unlikely event of a blackout. Only CTAC trained personnel or visitors accompanied by CTAC trained personnel may enter the SPF facility and visitors must sign-in upon entering.

Information Security

Cyagen is committed to the delivery of a first-class confidential service. Information pertaining to clients’ projects is treated in a strictly confidential manner. All information is stored on company servers in secured areas with daily backups. All systems are password protected with access restricted to authorized personnel only. Project information from clients is never shared with other clients or third parties. After a project is initiated, the project tracking number is used in place of information that can be used to identify the client during the production process. Only authorized personnel such as project managers have access to original customer information.

Animal Husbandry

Housing

Animals are housed in individual ventilated cage (IVC) systems. Each rack can accommodate 70-100 cages depending on the type of rack. Each cage houses 1-5 adult mice. For pre-weaning pups, the pups and female mice are housed in the same cage. Mice showing signs of fighting are separated into different cages.

Ventilation

Air in the facility is controlled by fully enclosed ventilation systems and is filtered to class 10,000. Different barrier areas in the facility are ventilated by independent systems. The air change rate is not less than 15 times per hour. Air source is 100% fresh (filtered from outside). Pressure in the clean corridor is >10 Pa higher than the animal housing areas, >10 Pa higher than the dirty corridors, and >10 Pa higher than the outside. Pressure gradients are monitored and recorded on a daily basis. Performance of the ventilation systems is certified by certificated Laboratory Animals Monitoring Institutions. Assessment is also conducted when problems arise (e.g. strong odor, rushing noise when doors are opened, stagnant air, etc.).

Temperature, Relative Humidity (RH) and Light-dark cycles (LD)

Temperature and relative humidity are individually controlled by a fully automatic air conditioning system. The temperature is maintained at 20-26°C. Relative humidity (RH) in animal rooms is maintained 40-70%. The Light-dark cycles (LD) is 12: 12, with the lights on from 6 am to 6 pm and dark for the rest of the time.

Personnel Gowning

Personnel allowed to enter the barrier facility wear a reusable full-body washable cover suit (covers the entire body from head to toe, except for the face and hands), sterilized disposable gloves, and surgical face masks. Full-body cover suits are washed and autoclaved after each use. Air showers are installed at entrances of some barrier areas. Prior to entering the air shower, workers put on appropriate cleanroom clothing in a gowning room.

Diet and Water

Pre-sterilized diets are purchased from certified suppliers that meet Chinese government standards (GB 14924.3-2010, "Experimental Animals: Nutrients in Formulated Feeds"). Diet quality is regularly monitored by state-approved third-party testing laboratories. Water is filtered from the city's public water system, put into bottles with straws, and autoclaved before use.

Bedding and Nesting Material

Bedding is purchased from a certified supplier, placed in cages, and they are autoclaved together before use. Additional enrichment items, such as nesting materials and shelter, are autoclaved before use.

Cage Change and Cage Washing

Cages are changed weekly at designated animal changing stations. In a typical animal changing station, laminar flow hood (LFH) receives high-efficiency particulate absorption (HEPA)-filtered air which, when supplied under positive pressure, protects animals in cages from airborne infections or other harmful particulate matter present in the environment. Dirty cages are automatically cleaned with cage washing machines and visually checked for cleanliness and visually inspected for cleanliness. Bedding is then added and the cages are autoclaved, with temperature sensitive labels placed inside selected cages to monitor the effectiveness of the sterilization.

Animal Monitoring

Animal care personnel observe animals to assess their health on a daily basis and document any signs of animal injury, illness, or death. Animals showing significant signs of pain or distress will be euthanized. Animals showing signs of mild to moderate pain or distress will be closely monitored and if these signs persist irreversibly, animals will be euthanized.