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Knockout Mice
With the capability to modify mouse genomes, genetically engineered knockout mice have become essential for modeling human diseases. At Cyagen, we support clients globally with diverse knockout mouse models, offering study-ready cohorts tailored to their research requirements.
Cost-Effective Solutions
High-quality gene targeting at unbeatable prices.
Swift Turnaround Time
Get your gene targeting mouse models faster.
Guaranteed Results
Get animals or your money back.
Overview
Workflow
FAQs
Overview
Comprehensive Gene Targeting Solutions
We provide comprehensive solutions for your gene targeting mouse model requirements, offering both conventional knockout and conditional knockout services. Whether you need assistance with the entire targeting project, from strategy design to mutant animal delivery, or prefer to outsource specific phases, we've got you covered. With the shortest turnaround time in the industry and unbeatable prices, trust us for efficient and cost-effective solutions tailored to your needs.

Our knockout mouse models offer invaluable resources for investigating gene function and modeling human diseases. Whether you require conventional knockout for permanent gene disruption or conditional knockout for tissue-specific targeting, we have the solution to meet your research needs.
Explore Ready-to-Use Mouse Models
Discover over 18,000 validated mouse strains—including knockout, conditional knockout, and humanized models—covering 20+ research areas such as oncology, neurology, and metabolism. All models are supported by detailed genotype data and guaranteed quality, helping you fast-track discovery with confidence.
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MouseAtlas Model Library
Search and access curated genetically engineered mouse strains
Turboknockout® Gene Editing
Fast-track your research with our efficient gene targeting service.
Targeted Gene Editing
AI-enhanced HDR technology for precise in vivo genome engineering
Rodent Breeding
Scalable colony expansion with full genotyping support
Cryopreservation & Recovery
Preserve and revive rodent strains on demand
Rodent Phenotyping
Full-spectrum analysis for rodents model
Workflow
Workflow and Delivery
Our streamlined process ensures efficient generation and delivery of knockout mouse models to meet your research timelines. Choose from our two advanced gene editing technologies, TurboKnockout® and Targeted Gene Editing, each offering unique advantages for precise gene targeting.
TurboKnockout® Technology
Targeted Gene Editing Technology
TurboKnockout® Technology
TurboKnockout® Technology
Explore our efficient TurboKnockout® process designed for precise and timely delivery of knockout mouse models.
Stage Description Turnaround Time
Design Collaborate on gene targeting strategy analysis and approval. 1-4 days
Vector Construction Construction and validation of targeting vector through sequencing and restriction digest. 6-8 weeks
Targeting in ES Cells Electroporation into TurboKnockout® ES cells (C57BL/6 or BALB/c), PCR screening, Southern blot, karyotyping. 8-14 weeks
Mouse Production Introduction of targeted ES cells into host embryos, optional breeding to F1, genotype verification. 18-20 weeks
Validation & Delivery Final phenotype and genotype validation of mice, delivery of study-ready cohorts. Depends on breeding choice
Note:
Pricing details are available upon inquiry. Optional services include extended breeding and specific strain requests. Turnaround times exclude institutional approval and shipping durations.
TurboKnockout® Technology
Targeted Gene Editing Technology
Leverage our cutting-edge Targeted Gene Editing technology for rapid and precise knockout mouse generation, designed to meet stringent research specifications.
Stage Description Turnaround Time
Design Provide the gene name for knockout; we design a nuclease-mediated strategy optimizing on-target activity and minimizing off-targets. 1-4 days
Injection Inject nuclease mRNA into fertilized mouse eggs, followed by implantation into surrogate mothers to produce founders. 10-14 weeks
Founder Screening Screen pups by PCR and sequencing to identify those with desired mutations; frameshift or critical exon deletions indicate knockouts.
Breeding Founders Optionally breed up to three founders to wildtype mice to obtain F1 mice carrying the knockout allele. 12-16 weeks
Validation & Delivery Validate knockout mice through genotyping and off-target analysis, and deliver study-ready cohorts. Depends on breeding
Note:
  • Pricing: Details are available upon inquiry for each service stage.
  • Strain Information: Services are primarily offered in the C57BL/6 strain, with options for other strains and rat models.
  • Turnaround Time Exclusions: Excludes time for obtaining host institution's approval for mouse importation and transit time during shipping.
FAQs
Frequently Asked Questions (FAQs)
What types of diseases or conditions are studied using knockout mice?
Knockout mice have been used in cancer, cardiovascular disease, neurodegenerative disorder, and immunology studies. Some examples include studying the role of tumor suppressor genes in cancer, investigating the molecular mechanisms of Alzheimer's disease, and exploring the immune response to viral infections.
What Customers Say About Cyagen
Violet Shimmer
Stanford University
The service provided to us by Cyagen is now in press at Nature as an article.
Scarlett Rouge
Seattle Children’s Hospital
We are very pleased with the state-of-the-art professional transgenic services provided by Cyagen for our study published recently in Nature. We continue to use Cyagen’s transgenic services as it allows us to do better and more efficient research with transgenic mice.
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