Regular Transgenic Mice

Transgenic mice: quite turnaround time and high expression level | Cyagen US Inc.

 

Transgenic mice have foreign DNA incorporated into the mouse genome and are used to study a wide range of gene functions and human diseases. We offer a one-stop solution for all your transgenic mouse model needs. You can contract us to perform your entire transgenic project from initial strategy design and DNA vector construction to transgenic animal delivery, or you can outsource specific phases of the project to us. Our turnaround time is the shortest in the industry, our prices are unbeatable, and for guaranteed services, we guarantee the generation of animals or your money back.

 

Workflow of Transgenic Mice Services

Each full transgenic project is split into the following phases:

workflow of transgenic mice services

1. Transgenic strategy design

  • Design a transgenic strategy that meets your research needs.
  • Develop a PCR genotyping strategy for founder screening.

2. Transgenic vector construction

  • Construct conventional plasmid-based transgenic vectors, or modify BACs by recombineering as needed for BAC-based transgenes (visit our BAC Modification Services page for details).
  • Confirm plasmid or BAC modification by restriction digest and sequencing as needed.

3. Pronuclear injection

  • Inject transgenic plasmid or BAC into the pronucleus of fertilized eggs.
  • Implant injected eggs into surrogate mothers to obtain offspring.

4. Transgenic mouse screening

  • Genotype pups by PCR to identify those carrying the transgene.

 

Types of Transgenic Mice Services

Preparation of DNA for injection and development of a genotyping strategy

The success of transgenic production depends critically on the quality of DNA used in pronuclear injection. Accordingly, we employ a special protocol to prepare top-quality DNA for our injections. Plasmid DNA is typically linearized for injection. BAC DNA is typically injected in unlinearized form, as this has been shown to produce similar biological outcomes as linearized BAC. A PCR-based genotyping strategy will be developed and tested. If you provide your own transgenic vector rather than having us construct it, you can send us either E. coli or unlinearized DNA in solution (≥50 µg total), and we will perform the necessary preparation to purify/linearize the DNA. We strongly discourage customers from sending us linearized, injection-ready DNA, because the quality of such DNA is often insufficient for pronuclear injection. If you provide your own vector without sequence information, you will also need to provide a PCR-based genotyping strategy along with your vector.

Generation of transgenic founder mice

- Plasmid or BAC injection to obtain founders, basic serviceA minimum of 400 eggs (C57BL/6) will be injected to produce live animals. This will result in an average of 2-3 transgenic founders in the case of plasmid injection or 1-2 founders in the case of BAC injection, but the actual numbers could fluctuate significantly and it is possible that no founders are obtained. If you wish to ensure the generation of founders, we strongly recommend that you use our guaranteed service option (see below).
 

- Plasmid or BAC injection to obtain founders, guaranteed service: We guarantee a minimum of 3 transgenic founders for plasmid injection or 2 founders for BAC injection (the actual number of founders obtained is typically higher).

 

- Genotyping pups to identify founders: Pups will be genotyped by PCR to identify founder mice.

 

Pricing and Turnaround Time

Service Donor egg strain Price Turnaround time
Transgenic strategy design   Free 1-4 days
Transgenic vector construction or BAC modification*   Please inquire 2-5 weeks
Prepare DNA for injection and develop genotyping strategy   $500 1-2 weeks
Plasmid injection to obtain founders, basic service C57BL/6 $3,450 6-10 weeks
Plasmid injection to obtain founders, guaranteed service C57BL/6 $3,950 6-16 weeks
BAC injection to obtain founders, basic service* C57BL/6 $4,450 8-14 weeks
BAC injection to obtain founders, guaranteed service* C57BL/6 $6,950 8-18 weeks
Genotyping pups to identify founders   $250 1 week
* If you need your BAC modified, click here to find out about BAC modification services.
Note: For transgenic mouse services not listed above, please inquire about availability and pricing. The turnaround time above does not include the time for obtaining host institution’s approval for mouse importation, nor transit time during shipping.
 

Donor Strain Information

We typically produce transgenic mice in C57BL/6 strain backgrounds, but we can use other strains per your request.  

>> Rat models are also available. Learn more about Regular Transgenic Rat Services.

 

Guarantee

Cyagen offers the best guarantee in the industry – we will fully refund the client’s service fee if animals with the specified genotype are not generated (except for genetic modifications severely affecting viability, morbidity, or fertility). Given the complexity of biological systems, a particular genetic modification may not result in the desired phenotype. As such, Cyagen's guarantee covers the creation of animals with the specified genotype, not a particular phenotypic outcome in terms of transcription, protein/RNA function, or organismal biology.

 

Order Supporting Information 

Please see below for supporting information related to Cyagen animal orders.

https://www.cyagen.com/us/en/support/order-information.html

 

Inquiries and Quote Requests

Request a quote now. Alternatively, you can always email animal-service@cyagen.com or call 800-921-8930 to inquire about our services or obtain a quote for your project.

 

Why work with Cyagen?

  • 14+ years' experience in custom animal model generation
  • 78,000+ animal models generated and delivered worldwide
  • 4,700+ citations in SCI journals for our services
  • 3,000+ university and company partners
  • 100% money-back guarantee option available for most services
  • AAALAC-Accredited and OLAW Assured