Cyagen's Year-End Savings: Save up to 10% on custom animal models
Cyagen's Year-End Savings Event
Custom Animal Model Capabilities
If you are seeking to generate a custom rodent model for your research project, it is the
perfect time to lock-in savings and ensure funds don’t go to waste.
Cyagen's Year-End Savings Event can help you extend your research with big savings for
multiple projects - save up to 10% off custom animal models for research. The more mouse
models you order, the more you save!
Pay Now – Start Later
We can initiate projects to meet your project timeline – so you can lock-in promotional
prices on multiple models and decide project details later. Our Model Design Specialists can
provide complimentary project strategy details within 24 hours and initiate your project as
soon or as late as you require.
Refer a colleague
Waiting on funding? Refer a colleague now to receive a coupon for these limited-time discounts
that you may apply when your grant arrives. Referral coupon will be provided after the referred
project is placed during the promotional period - before December 18, 2020. To refer a
colleague, simply send us an email at
animal-service@cyagen.com
with a brief statement
indicating whom is being referred (please copy all referred colleagues).
Cyagen's Year-End Savings Event Details
●Promotion Period:
Offer ends December 18, 2020.
●Eligibility:
Academic end clients in Europe, North America, and South America. Promotional pricing is only
available for new projects with new clients and may not be applied to past inquiries or projects
in-progress.
The offer applies to the following eligible custom mouse and rat model services:
Custom Mouse and Rat Model Services
Model Generation Services
Species
1 Project
≥2 Projects
Deliverables
ES Cell Mediated
TurboKnockout
Mouse only
5% off
10% off
≥3 F1 animals
CRISPR/Cas - based
Knockout (KO)
Mouse or Rat
5% off
10% off
≥3 F1 animals
KI (any locus)
KI (ROSA26)
Point mutation
cKO
Transgenic
Regular Transgenic
Mouse or Rat
5% off
≥3 founders (plasmid injection) or ≥2 founders (BAC injection)
PiggyBac Transgenic
PiggyBac-on-BAC Transgenic
Note
① Any of the listed services may count towards the total discounts received, up to 10% off
per project. Transgenic services are only eligible for 5% off the list price but may count
towards the total number of projects - increasing discounts for all other eligible services
listed.
② Listed prices do not include handling fees or Taconic's RapidCheck Health Certification
Program.
About Cyagen
Cyagen's experts have generated thousands of models and offer 100% money back guarantee on standard
projects - no animals, no charge.
TurboKnockout® - Proprietary Method for Accelerated Generation of Precise Mouse Models
Cyagen's Proprietary Method for Accelerated Generation of Precise Mouse Models
Our proprietary TurboKnockout® gene targeting method can provide you with conditional
knockout, reporter knockin and humanization mouse models as fast as 6-8 months. This proprietary
gene targeting method is enabled by two innovations:
● Eliminates the unpredictability of germline
transmission (GLT)with our super competent ESC line that generates 100% ESC-derived founder mice
rather than chimeras.
● Self-removing selection cassette circumvents the need
to breed to Flp deleter mice.
These innovations eliminate at least two generations of breeding, shortening production time by
4-6 months as compared to the industry standard.
Faster Turnaround with Turboknockout®
TurboKnockout® gene targeting: 6-8 months
Targeting Vector Construction
2 months
Gene Targeting in ES Cells
2-3 months
Founder Het Generation
2-3 months
Traditional gene targeting: 10-14 months
Targeting Vector Construction
2 months
Gene Targeting in ES Cells
2-3 months
Chimera Generation
2-3 months
F1 Het Generation
2-3 months
Removal of Selection Cassette
2-3 months
Highlights of TurboKnockout® Gene Targeting
●No patent risk:
Technology of choice for drug development projects ●Success rate:
100% guaranteed germline transmission (GLT) ●Turnaround time:
Founders as fast as 6-8 months ●Large fragment knockin size:
Knockin (KI) fragment size up to 200 kb ●Limitless loxP:
No size limitations for your floxed region ●100% Money-Back Guarantee on TurboKnockout® projects
Capabilities
Donor Strains Mouse: C57BL/6, BALB/c
TurboKnockout® for BALB/c Drug Development Models
BALB/c is a laboratory-bred, albino strain of the house mouse, characterized by its
immunodeficiency and extreme susceptibility to carcinogens. BALB/c mice are among the most
widely used mouse strains across oncology, immunology, nuclear medicine research, and are often
the first option for animal experimentation models in new drug development projects.
TurboKnockout® is based on traditional embryonic stem cell (ESC) targeting
techniques, which can be used for complex genetic modification projects to provide BALB/c mouse
models and is the technology of choice among our clients performing drug development projects.
Cyagen CRISPR-Pro Technology - Capabilities for Mouse & Rat Models
Engineered nuclease-mediated genome editing (especially CRISPR/Cas) is an emerging technology
which can serve as an alternative to the conventional, ES cell homologous recombination-based
knockout (KO) animal generation. These methods have enabled the generation of custom mouse and
rat models with genetic knockouts, point mutations, and large fragment knockins (LFKIs).
Cyagen's CRISPR-based Rapid Knockout services use accelerated methodology that provides
conventional knockout, reporter knockin, and humanization founder animals in as fast as 3
months.
Capabilities
Donor Strain Information:
●
Mouse strains: C57BL/6, FVB ●
Rat strains: Sprague-Dawley (SD), Long Evans
Note: Other strains available upon request.
Transgenic Capabilities for Animal Models
Items
Regular Transgenic
PiggyBac Transgenic
Rosa26 Targeted Transgenic
Integration
Random, multicopy integration
Random, single copy per integration site
Single copy transgene targeted to Rosa26 safe harbor locus
Vector construction
Plasmid or BAC
Plasmid or BAC
CRISPR or TurboKnockout®
Expression pattern
Variable
More consistent
Most consistent
Endogenous effects
Can disrupt endogenous genes
Less likely to disrupt endogenous genes
Safe harbor locus
Donor Strain Information
Mouse strains: C57BL/6, FVB
Rat strains: Sprague-Dawley (SD), Long Evans
Note: Other strains available upon request.
PiggyBac transgenic
services can deliver single-copy transgenic animals with more consistent
expression of your transgene. This proprietary transgenic method has the following advantages
over other transgenic approaches:
●Single-copy integration:
Avoids potential gene silencing from multiple copies ●Defined region of integration:
No loss of transgene sequence (TTAA, transcription unit) ●Reliability:
More consistent expression pattern compared to plasmid based transgenics ●Economical:
Cost and turnaround time comparable to plasmid based transgenics
Cyagen Model Generation Expertise
14+
years' experience in custom animal model generation
50,000+
animal models generated and delivered worldwide
4,000+
citations in SCI journals for our services
3,000+
university and company partners
Customer Testimonials
"We generated a mouse knockout model using Cyagen’s TurboKnockout® technology.
Cyagen's team was on time with milestones, provided constant updates on the project and
delivered animals as promised. It was such a pleasure working with Cyagen that we decided to
outsource the production of other animal models to them."
-- Maximiliano D’Angelo, Sanford Burnham Prebys Medical Discovery Institute
Contact us for a free technical consultation and quote.
We use cookies to ensure that we give you the best experience on our website. To learn about how we
keep your information safe, view our Privacy Policy.
OKRejectPrivacy policy
Customer Testimonials
