Transgenic Mouse Embryos

Transgenic mice embryos have foreign DNA incorporated into the mouse genome and are used to study a wide range of gene functions and human diseases. We offer a one-stop solution for all your transgenic mouse embryos needs. You can contract us to perform your entire transgenic mouse embryo project from initial strategy design and DNA vector construction to transgenic mouse embryo delivery, or you can outsource specific phases of the project to us. Our turnaround time is the shortest in the industry, our prices are unbeatable, and for guaranteed services, we guarantee the generation of embryos or your money back. 

Workflow of Transgenic Mouse Embryos Services

Each full transgenic mouse embryos project is split into the following phases:

1. Transgenic strategy design

• Design a transgenic strategy that meets your research needs.
• Develop a PCR genotyping strategy for mouse embryos screening.

2. Transgenic vector construction

• Construct conventional plasmid-based transgenic vectors, or modify BACs by recombineering as needed for BAC-based transgenes (visit our DNA Vector Construction & BAC Modification Services page for details).
• Confirm plasmid or BAC modification by restriction digest and sequencing as needed.

3. Pronuclear injection

• Inject transgenic plasmid or BAC into the pronucleus of fertilized eggs.
• Implant injected eggs into surrogate mothers to obtain offspring.

4. Transgenic mouse embryos screening and LacZ staining

• Embryos harvested at the desired embryonic stage will be genotyped by PCR and lacZ stained to identify the ones carrying the desired transgene.

Types of Transgenic Mouse Embryos Services

Preparation of DNA for injection and development of a genotyping strategy

The success of transgenic production depends critically on the quality of DNA used in pronuclear injection. Accordingly, we employ a special protocol to prepare top-quality DNA for our injections. Plasmid DNA is typically linearized for injection. BAC DNA is typically injected in unlinearized form, as this has been shown to produce similar biological outcomes as linearized BAC. A PCR-based genotyping strategy will be developed and tested. If you provide your own transgenic vector rather than having us construct it, you can send us either E. coli or unlinearized DNA in solution (≥50 µg total), and we will perform the necessary preparation to purify/linearize the DNA. We strongly discourage customers from sending us linearized, injection-ready DNA, because the quality of such DNA is often insufficient for pronuclear injection. If you provide your own vector without sequence information, you will also need to provide a PCR-based genotyping strategy along with your vector.

Generation of transgenic mouse embryos and staining of embryos for lacZ expression

- Plasmid or BAC injection to obtain embryos, basic service: A minimum of 150 eggs (C57BL/6 strain) will be injected. Embryos will be retrieved from surrogate mothers at the stage you specify (typically within the E10.5-E16.5 window) for subsequent processing. This will result in an average of 2-3 transgenic embryos, but the actual numbers could fluctuate significantly and it is possible that no transgenic embryos are obtained. If you wish to ensure the generation of transgenic embryos, we strongly recommend that you use our guaranteed service option (see below).

- Plasmid or BAC injection to obtain embryos, guaranteed service: We guarantee a minimum of 4 transgenic embryos for plasmid injection or 2 transgenic embryos for BAC injection (the actual number of transgenic embryos obtained is typically higher).

- Genotyping and lacZ staining of embryos: Embryos will be genotyped by PCR. All embryos, regardless of genotype, will also be fixed and stained for lacZ expression.

Pricing and Turnaround Time

Guarantee

Cyagen offers the best guarantee in the industry – we will fully refund the client’s service fee if animals with the specified genotype are not generated (except for genetic modifications severely affecting viability, morbidity, or fertility). Given the complexity of biological systems, a particular genetic modification may not result in the desired phenotype. As such, Cyagen's guarantee covers the creation of animals with the specified genotype, not a particular phenotypic outcome in terms of transcription, protein/RNA function, or organismal biology.

Order Supporting Information 

Please see below for supporting information related to Cyagen animal orders.

（https://www.cyagen.com/us/en/support/order-information.html）

Inquiries and Quote Requests

Request a quote now. Alternatively, you can always email animal-service@cyagen.com or call 800-921-8930 to inquire about our services or obtain a quote for your project.