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Citations
IMMUNITY 47:107 (2017) IF=22.845
Ctsk-iCre Mice
Catalog Number：C001366
Genetic Background： C57BL/6J
Reproduction：Carrier x Carrier
Strain Description
The Ctsk gene encodes a protein that is a lysosomal cysteine protease involved in bone remodeling and resorption. This protein is a member of the peptidase C1 protein family and is primarily expressed in osteoclasts. When Ctsk-iCre mice are crossed with mice containing loxP sites, the offspring can generate sequence deletions between loxP sites mediated by Cre recombinase in osteoclasts. The heterozygous Ctsk-iCre mice are viable and fertile.
The TGA stop codon was replaced with the “P2A-iCre” cassette. The co-expression of Ctsk and Cre can be achieved.
a. Method
The Ctsk-iCre mice were bred with Rosa26-LSL-tdTomato mice to generate double heterozygous offspring. Cre-mediated recombination will result in the expression of tdTomato protein in the Cre-positive cells of the offspring. The bone tissue was collected from the offspring mice and immunofluorescence was used to observe the expression of tdTomato protein to determine the activity of Cre recombinase.
b. Genotype
Cre+: Ctsk-iCre[KI/+]; Rosa26-LSL-tdTomato[CKI/+]；
Cre-: Rosa26-LSL-tdTomato[CKI/+]
c. Result
Figure 1. Immunofluorescence of the bone tissues. The results showed that the tdTomato red fluorescence was localized and overlapped with TRAP staining in osteoclasts, indicating that Cre recombination occurred in these cells.
In summary, this model shows significant fluorescence recombination effects in osteoclasts.
The Cre recombinase gene is located on chromosome 3 in Ctsk-iCre mice. Please avoid using mice that have been targeted on the same chromosome for breeding.
