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Citations
IMMUNITY 47:107 (2017) IF=22.845
You may also be interested in
H11-Vav1-iCre Mice
Catalog Number：C001357
Genetic Background：C57BL/6J
Reproduction：Cre+/+ x Cre+/+or Cre+/-x Cre+/-
Strain Description
The vav guanine nucleotide exchange factor 1 (VAV1) gene is a member of the VAV gene family, and the proto-oncogene vav proteins encoded by it are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. The VAV protein is a member of the Dbl family of guanine nucleotide exchange factors (GEF) for the Rho family of GTP binding proteins. The protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation.
This strain carries a mouse Vav1 gene promoter-driven improved Cre (iCre) expression element insertion. When H11-Alb-iCre mice are crossed with mice containing loxP sites, Cre recombinase-mediated loxP site sequence recombination is induced in the hematopoietic stem cells (HSCs) of the offspring. The homozygous H11-Vav1-iCre mice developed normally and were fertile.
The Vav1 Promoter-iCre gene expression element was inserted into the H11 safety harbor in C57BL/6J mice.
Detection of Cre expression
a. Method
H11-Vav1-iCre mice were mated with Rosa26-LSL-tdTomato mice to produce double heterozygous offspring mice. Peripheral blood (PB), spleen, and bone marrow (BM) of the offspring mice were collected and the fluorescence occurrence was observed by flow cytometry to confirm the expression of Cre protein.
b. Result
Cre+: H11-Vav1-iCre[KI/+];Rosa26-LSL-tdTomato[CKI/+]
Cre-: Rosa26-LSL-tdTomato[CKI/+]
Description: Strong tdTomato fluorescence signals were detected in CD45+ hematopoietic stem cells (HSCs) of bone marrow (BM) and spleen, and fewer signals were detected in peripheral blood (PB) CD45+ cells.
c. Conclusion
This strain presents strong Cre-mediated recombination in CD45+ hematopoietic stem cells.
The transgenic element of this strain is located on chromosome 11, please avoid using flox mice with the transgenic locus on chromosome 11 for breeding.
