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Citations
IMMUNITY 47:107 (2017) IF=22.845
You may also be interested in
SD-CAG-EGFP Rats
Catalog Number: CR002
Genetic Background: Crl:CD(SD)
Reproduction: Carrier x WT
Strain Description
This strain represents a transgenic rat harboring a transgene encoding the enhanced green fluorescent protein (EGFP). EGFP expression is modulated by the CAG promoter [1]. The CAG promoter, a synthetic promoter extensively utilized in molecular biology to achieve robust gene expression, constitutes a fusion of the cytomegalovirus (CMV) early enhancer element and the chicken beta-actin promoter. This promoter is renowned for its robust constitutive expression across a diverse array of tissues and cell types [2-3].
In this strain, the CAG promoter drives the expression of EGFP throughout the rat body. The EGFP protein can be directly observed under a fluorescent microscope when excited by light. Validation data show that EGFP protein is expressed in the brain, cerebellum, olfactory bulb, heart, thymus, lungs, liver, spleen, stomach, large intestine, small intestine, kidneys, pancreas, uterus, ovaries, adipose tissue, muscle, toes, tail, and ears of SD-CAG-EGFP rats. Therefore, SD-CAG-EGFP rats can be used for research in areas such as the labeling and tracking of rat cells or tissues. In addition, cells isolated from SD-CAG-EGFP rats may be easier to culture than wild-type rat primary cells that have been transfected with EGFP plasmids.
Strain Strategy
Transgenic Crl:CD(SD) rat embryos were obtained by injecting the linearized CAG-EGFP plasmid fragment into the pronucleus of Crl:CD(SD) rat embryos. Rats that show EGFP fluorescence throughout the body were selected as founder rats. The SD-CAG-EGFP rat strain was established by breeding the founder rats with wild-type Crl:CD(SD) rats.
Application
SD-CAG-EGFP rats can be used for cell or tissue labeling and tracking research in research areas such as developmental biology, cell biology, immunology, and neuroscience.
Validation Data
1. The expression of EGFP protein in the whole body tissues and organs of SD-CAG-EGFP rats
Figure 1. Expression of enhanced green fluorescent protein (EGFP) in SD-CAG-EGFP rats. Tissues and organs from SD-CAG-EGFP rats, including the brain, cerebellum, olfactory bulb, heart, thymus, lungs, liver, spleen, stomach, large intestine, small intestine, kidneys, pancreas, uterus, ovaries, adipose tissue, muscle, toes, tail, and ears, were observed under a microscope for the occurrence of green fluorescence. The results showed that strong green fluorescence signals were present in almost all tissues of SD-CAG-EGFP rats.
Announcement
This strain was generated using transgenic technology, with the integration site of the EGFP transgene remaining unknown. As a result, conventional PCR can only confirm the presence of the EGFP transgene, but cannot distinguish between homozygous and heterozygous rats.
References
[1]Phillips GJ. Green fluorescent protein--a bright idea for the study of bacterial protein localization. FEMS Microbiol Lett. 2001 Oct 16;204(1):9-18.
[2]Okabe M, Ikawa M, Kominami K, Nakanishi T, Nishimune Y. 'Green mice' as a source of ubiquitous green cells. FEBS Lett. 1997 May 5;407(3):313-9.
[3]Li S, Chen LX, Peng XH, Wang C, Qin BY, Tan D, Han CX, Yang H, Ren XN, Liu F, Xu CH, Zhou XH. Overview of the reporter genes and reporter mouse models. Animal Model Exp Med. 2018 Apr 19;1(1):29-35.
