Catalog Number: I001181
Strain Name: C57BL/6JCya-Mapttm2(hMAPT*P301L)/Cya
Genetic Background: C57BL/6JCya
Reproduction: Homozygote x Homozygote
One of Cyagen’s HUGO-GTTM (Humanized Genomic Ortholog for Gene Therapy) Strains
Frontotemporal Dementia (FTD) is the second most prevalent form of early-onset dementia, following Alzheimer’s disease (AD). This condition is distinguished by the selective degeneration of the frontal and temporal lobes, resulting in personality and behavioral changes, language impairments, and executive dysfunction. Approximately 40%-50% of FTD cases have a familial component, with known causative genes including MAPT, FUS, and TARDBP. Of these, MAPT is the earliest discovered and most frequently implicated in FTD, mutations in the MAPT gene are detectable in roughly 30% of familial FTD cases [1].
The tau protein, a microtubule-associated protein encoded by MAPT is primarily localized to neuronal axons and plays a critical role in microtubule stability and assembly. By binding to microtubules, tau protein helps to maintain neuronal cell shape. Mutations in MAPT can promote tau aggregation, leading to pathological tau protein accumulation and death of glutamatergic cortical neurons [2]. Additionally, certain MAPT mutations can affect pre-mRNA exon splicing, altering the ratio of 3R to 4R tau protein isoforms and increasing the relative production of 4R-tau protein, which is more prone to fibril formation. Common mutations include P301L, P301S, and Intron10+3 G>A [4]. The P301L mutation affects the 4R-tau isoforms without affecting splicing in exon 10. This mutation accelerates the formation of paired helical filaments in tau proteins, reduces microtubule interactions and stability, and promotes β-sheet folding during the aggregation process. This leads to abnormal tau protein aggregation, resulting in neurofibrillary tangles—a characteristic feature of neurodegenerative diseases [11-12].
Therapies targeting the MAPT gene primarily consist of small molecule drugs and monoclonal antibodies, with indications including AD and FTD. Transgenic mice are frequently used in drug development, and the utilization of humanized animal models helps advance potential MAPT-related therapies toward clinical trials. This strain is a mouse Mapt gene humanized model carrying P301L mutation and can be used for research on FTD and AD. The homozygous B6-htau*P301L mice are viable and fertile. In addition, based on the independently developed TurboKnockout fusion BAC recombination technology, Cyagen can also generate other hot mutation models (e.g., B6-htau*P301S mice) and provide customized services for specific mutations.
Figure 1. Gene editing strategy of B6-htau*P301L mice. The mouse Mapt gene was replaced with the human MAPT gene carrying the P301L mutation by gene editing technology.
Figure 2. RT-qPCR detection of human MAPT gene and mouse Mapt gene expression in the liver, cerebral cortex, and kidney of 6-week-old female B6-htau*P301L mice (hMAPT*P301L), B6-htau (hMAPT) and wild-type (WT) mice. The results indicate that B6-htau*P301L mice and B6-htau mice significantly express the human MAPT gene in the liver, cerebral cortex, and kidney, while not expressing the mouse Mapt gene. In contrast, WT mice only exhibit expression of the mouse Mapt gene and do not show expression of the human MAPT gene.
(ND: Not detected)
2. Behavioral Testing: Open Field Test
a. 3-month-old
Figure 3. The travel distance (A-C) and central area time ratio (D-F) for WT, hMAPT WT, hMAPT-P301L, and hMAPT-P301S mice in open field test. Data were analyzed using an ordinary one-way ANOVA; "ns" indicates no significant differences, **p < 0.01.
Indications:
(A-C) No significant alterations in travel distance across all models.
(D-F) A notable decrease in the time spent in the central area is observed in hMAPT-P301S female when compared to their hMAPT WT counterparts, suggesting a slight increase in anxiety levels.
b. 6-month-old
Figure 4. The travel distance (A-C) and central area time ratio (D-F) for WT, hMAPT WT, hMAPT-P301L, and hMAPT-P301S mice in open field test. Data were analyzed using an ordinary one-way ANOVA; "ns" indicates no significant differences, *p < 0.05, **p < 0.01.
Indications:
(A-C) In comparison to WT mice, the hMAPT WT, hMAPT-P301S, and hMAPT-P301L exhibited a significant rise in travel distance. Given that no differences were noted among the three HUGO strains, we suggest that this increase in travel distance is likely a physiological characteristic rather than a pathological change (e.g. hyperactivity).
(D-F) No significant alterations in central area time ratio across all models.
3. Behavioral Testing: Y Maze Test
a. 3-month-old
Figure 5. The travel distance (A-C) and ratio of spontaneous alterations (D-F) for WT, hMAPT WT, hMAPT-P301L, and hMAPT-P301S mice in Y maze test. Data were analyzed using an ordinary one-way ANOVA; "ns" indicates no significant differences.
Indications:
(A-C) No significant alterations in travel distance across all models.
(D-F) No significant alterations in the ratio of spontaneous alterations across all models.
b. 6-month-old
Figure 6. The travel distance (A-C) and ratio of spontaneous alterations (D-F) for WT, hMAPT WT, hMAPT-P301L, and hMAPT-P301S mice in Y maze test. Data were analyzed using an ordinary one-way ANOVA; "ns" indicates no significant differences, *p < 0.05.
Indications:
(A-C) No significant alterations in travel distance across all models.
(D-F) When comparing males and females, there were no notable differences in the ratio of spontaneous alterations. However, in sex-mixed comparisons, the larger group size led to a slight decrease in the ratio of spontaneous alterations for hMAPT WT, hMAPT-P301S, and hMAPT-P301L. Since no differences were observed among the three HUGO strains, we believe that the decrease in the ratio of spontaneous alterations is probably a physiological trait rather than an indication of a pathological change, such as impaired spatial memory.
4. Behavioral Testing: Rotarod Test
a. 3-month-old
Figure 7. The latency for WT, hMAPT WT, hMAPT-P301L, and hMAPT-P301S mice in rotarod test. Data were analyzed using an ordinary one-way ANOVA; "ns" indicates no significant differences.
Indications:
No significant alterations in the latency across all models.
b. 6-month-old
Figure 8. The latency for WT, hMAPT WT, hMAPT-P301L, and hMAPT-P301S mice in rotarod test. Data were analyzed using an ordinary one-way ANOVA; "ns" indicates no significant differences.
Indications:
No significant alterations in the latency across all models.
5. Behavioral Testing: Novel Object Recognition Test
a. 6-month-old, Male
Figure 9. The object preference (percentage of exploring the specific object/total exploration time of both objects) for male WT, hMAPT WT, hMAPT-P301L, and hMAPT-P301S mice in novel object recognition test. Data were analyzed using an ordinary one-way ANOVA; "ns" indicates no significant differences, *p < 0.05, **p < 0.01.
Indications:
For the male comparison, WT, hMAPT WT, and hMAPT-P301L displayed a strong tendency to investigate new objects. In contrast, hMAPT-P301S mice exhibited almost no variation in their exploration patterns between familiar and novel objects, implying a considerable deficit in episodic memory.
b. 6-month-old, Female
Figure 10. The object preference (percentage of exploring the specific object/total exploration time of both objects) for female WT, hMAPT WT, hMAPT-P301L, and hMAPT-P301S mice in novel object recognition test. Data were analyzed using an ordinary one-way ANOVA; "ns" indicates no significant differences, *p < 0.05, **p < 0.01.
Indications:
For the female comparison, WT and hMAPT WT displayed a strong tendency to investigate new objects. In contrast, hMAPT-P301L mice showed a minor decrease in their preference for exploring new objects, resulting in an insignificant distinction between the familiar and novel subjects, indicating a slight impairment in episodic memory. Meanwhile, hMAPT-P301S mice exhibited almost no variation in their exploration patterns between familiar and novel objects, implying a considerable deficit in episodic memory.
c. 6-month-old, Male & Female
Figure 11. The object preference (percentage of exploring the specific object/total exploration time of both objects) for mixed-sex WT, hMAPT WT, hMAPT-P301L, and hMAPT-P301S mice in novel object recognition test. Data were analyzed using an ordinary one-way ANOVA; "ns" indicates no significant differences, *p < 0.05, ***p < 0.001.
Indications:
WT and hMAPT WT mice exhibited a strong tendency to investigate unfamiliar objects. In contrast, the previously observed lack of this inclination in hMAPT-P301L mice became evident in a mixed-sex setting, potentially due to the increased group size. Meanwhile, hMAPT-P301S mice showed minimal to no variation in their exploration patterns between familiar and novel objects, indicating a notable deficit in episodic memory.
Frontotemporal Dementia (FTD) is the second most prevalent form of early-onset dementia, following Alzheimer’s disease (AD). FTD typically exhibits an autosomal dominant inheritance pattern. Pathologically and radiographically, FTD is characterized by selective degeneration of the frontal and temporal lobes, resulting in personality and behavioral changes, language impairments, and executive dysfunction. Approximately 40%-50% of FTD cases have a familial component, with known causative genes including MAPT, FUS, and TARDBP. Of these, MAPT is the earliest discovered and most frequently implicated in FTD, mutations in the MAPT gene are detectable in roughly 30% of familial FTD cases.
The human MAPT gene is located on chromosome 17 and encodes the microtubule-associated tau protein. Tau protein is primarily localized to neuronal axons and plays a critical role in microtubule stability and assembly. Mutations in MAPT can lead to pathological tau protein accumulation and neuronal death in glutamatergic cortical neurons. These mutations, typically occurring in exons 9-12 and adjacent intronic regions, fall into two main categories: The first type affects protein expression, altering the protein’s structure and stability. Deletion of the MAPT gene may impair its function and exacerbate abnormal tau protein aggregation, consistent with the acquisition of cytotoxic effects. Similarly, mutations at specific sites can increase the propensity of tau protein to aggregate. The second type affects pre-mRNA exon splicing, altering the ratio of 3R to 4R tau protein isoforms and increasing the relative production of 4R-tau protein, which is more prone to fibril formation. Common MAPT mutations include P301L, P301S, and Intron10+3 G>A, among others [4]. Unlike the P301S mutation, P301L does not affect the splicing of exon 10. This mutation accelerates the formation of paired helical filaments in tau proteins, reduces microtubule interactions and stability, and promotes β-sheet folding during the aggregation process [11-12]. This leads to abnormal tau protein aggregation, resulting in neurofibrillary tangles—a characteristic feature of neurodegenerative diseases. Similar to the P301L mutation, the P301S mutation also causes pathological tau protein aggregation. Research indicates that recombinant tau proteins carrying the P301S mutation significantly impair microtubule assembly [13], and patients with this mutation exhibit clinical heterogeneity [14].
According to the publications, the pathogenic Intron10+3 G>A mutation in the MAPT intron can result in an increased proportion of 4R isoforms. Treatment with the oligonucleotide drug ASO-001933, which targets the 3’UTR region of MAPT, has been shown to effectively reduce tau protein expression in mice [5], non-human primates, and primary cultures of human neurons [6].
Therapies targeting the MAPT gene primarily consist of small molecule drugs and monoclonal antibodies, with indications including AD and FTD. Transgenic mice are frequently used in drug development, and the utilization of humanized animal models helps advance potential MAPT-related therapies toward clinical trials. The ASO drug ISIS-814907 from Ionis targets and reduces MAPT gene expression to treat disease. Preclinical research for this drug candidate utilized transgenic humanized PS19 mice, in which the human MAPT gene carrying the P301S mutation was randomly inserted [7-8]. ASO-001933 targets the 3’UTR region of MAPT and effectively reduces its expression, In one study, a humanized disease model obtained by crossing transgenic mice (randomly inserting cDNA of the human wild-type MAPT gene) with MAPT-KO mice was used to pharmacologically analyze [5]. This study was funded by Roche [6]. Additionally, Arvinas’ ASO molecule and monoclonal antibody drug targeting the MAPT gene are currently in preclinical research [9].
The MAPT gene is a crucial pathogenic gene associated with frontotemporal dementia (FTD). Currently, gene therapy primarily focuses on antisense oligonucleotides (ASOs) and utilizes humanized mouse models for preclinical drug testing. Notably, MAPT gene humanized mice from Cyagen, along with popular mutation disease models (such as B6-htau*P301L and B6-htau*P301S mice), can be applied in preclinical research for FTD gene therapy. Cyagen also offers customized services for different mutations.
References
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[3]Lisowiec J, Magner D, Kierzek E, Lenartowicz E, Kierzek R. Structural determinants for alternative splicing regulation of the MAPT pre-mRNA. RNA Biol. 2015;12(3):330-42.
[4]Molecular Genetics Department, University of Antwerp. AD Mutations. http://www.molgen.vib-ua.be/ADMutations
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[8]Yoshiyama Y, Higuchi M, Zhang B, Huang SM, Iwata N, Saido TC, Maeda J, Suhara T, Trojanowski JQ, Lee VM. Synapse loss and microglial activation precede tangles in a P301S tauopathy mouse model. Neuron. 2007 Feb 1;53(3):337-51.
[9]Arvinas. (2021). Arvinas 2021 Investor Day Presentation. https://ir.arvinas.com/static-files/e04cc75d-eaf0-4b83-8b7a-68537fe79dc8
[10]Boyarko B, Hook V. Human Tau Isoforms and Proteolysis for Production of Toxic Tau Fragments in Neurodegeneration. Front Neurosci. 2021 Oct 21;15:702788.
[11]Barghorn S, Zheng-Fischhöfer Q, Ackmann M, Biernat J, von Bergen M, Mandelkow EM, Mandelkow E. Structure, microtubule interactions, and paired helical filament aggregation by tau mutants of frontotemporal dementias. Biochemistry. 2000 Sep 26;39(38):11714-21.
[12]Alzforum. (2021). MAPT P301L Mutation. Retrieved from https://www.alzforum.org/mutations/mapt-p301l
[13]Bugiani O, Murrell JR, Giaccone G, Hasegawa M, Ghigo G, Tabaton M, Morbin M, Primavera A, Carella F, Solaro C, Grisoli M, Savoiardo M, Spillantini MG, Tagliavini F, Goedert M, Ghetti B. Frontotemporal dementia and corticobasal degeneration in a family with a P301S mutation in tau. J Neuropathol Exp Neurol. 1999 Jun;58(6):667-77.
[14]Yasuda M, Nakamura Y, Kawamata T, Kaneyuki H, Maeda K, Komure O. Phenotypic heterogeneity within a new family with the MAPT p301s mutation. Ann Neurol. 2005 Dec;58(6):920-8.