CRISPR-Pro-based services

Cyagen’s CRISPR-based gene targeting services can generate custom mice and rats – including conventional knockout, conditional knockout, large-fragment knockin (LFKI), and humanization models - in as fast as 3 months.

What Are the Advantages of CRISPR-Pro-based Technology?

Compared with other gene editing technologies, the key advantages of CRISPR-Pro are its high efficiency, rapid turnaround, low cost, convenient process, and successful application to different species. CRISPR-Pro significantly reduces turnaround time compared with traditional embryonic stem (ES) cell-mediated gene targeting due to its direct application in embryo. Our AI-based bioinformatics tools, such as AlphaKnockout, rapidly identify the optimal sequences for guide RNAs (gRNAs) to minimize off-target effect and guarantee the desired genotype is successfully introduced. 

Cyagen's CRISPR-Pro Rodent Model Generation Capabilities

Cyagen's Innovations in CRISPR-Pro - Enhanced Targeting Efficiency

As Cas can be programmed to make DNA double-strand breaks (DSBs) in the genome of cells, these breaks are then repaired by the cells’ DNA damage repair mechanisms, such as non-homologous end joining (NHEJ) or homologous recombination (HR). However, NHEJ-mediated repair of Cas-generated breaks can generate small insertions and deletions at the targeted site during double-stranded break (DSB) repair. Cyagen’s CRISPR technology offers a solution to the limitations presented by the occurrence of NHEJ - our unique fertilization procedure increases the proportion of DSBs repaired by HR. This technique has greatly improved the accuracy and efficiency of CRISPR-based gene modifications and enabled more complicated models, including large fragment knockin (up to 15kb) and conditional knockouts.