Knockin Mice

Cyagen's knockin mice offer precise genetic modifications tailored to diverse research needs, including humanized and large-fragment knockins across C57BL/6 or BALB/c strains. These models enable effective disease modeling and functional studies by replicating human gene functions within a mouse, providing reliable, study-ready cohorts to fast-track preclinical research.

Guaranteed Results

Get animals or your money back.

Rapid Model Creation

Fast, precise genetic edits with guaranteed germline transmission.

Freedom to Operate

Patent dispute-free, enhancing drug development efficiency.

Overview

Workflow

FAQs

Overview

Precision Knockin Mouse Models

Cyagen offers precision-engineered knockin mouse models for advanced human gene function and disease research. Our comprehensive services range from point mutations to large-fragment knockins using state-of-the-art TurboKnockout® and Targeted Gene Editing technologies. Whether targeting the safe harbor locus (ROSA26/H11) or conducting any locus knockins, our models are tailored to enhance gene function studies and disease modeling in strains like C57BL/6 and BALB/c.

Trust Cyagen for efficient, cost-effective, and reliable knockin mouse model generation. We specialize in a broad range of project capabilities, handling knockin projects involving large fragments up to 300 kb. Each model is meticulously tailored to provide consistent and predictable results, ensuring alignment with your specific research needs.

Explore Ready-to-Use Mouse Models

Discover over 18,000 validated mouse strains—including knockout, conditional knockout, and humanized models—covering 20+ research areas such as oncology, neurology, and metabolism. All models are supported by detailed genotype data and guaranteed quality, helping you fast-track discovery with confidence.

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Workflow

Workflow and Delivery

Our refined workflow ensures the precise and timely creation of knockin mouse models tailored to your research specifications. Utilize our advanced TurboKnockout® and Targeted Gene Editing technologies to achieve unparalleled accuracy and efficiency in gene targeting, facilitating faster transitions from design to delivery.

TurboKnockout® Technology for Knockin Mice

Explore our efficient TurboKnockout® process tailored for the precise delivery of knockin mouse models. Here's an outline of our workflow:

Stage	Description	Turnaround Time
Gene Targeting Strategy Design	Analyze target gene structure, adjacent genes, and existing models. Develop screening methods for targeted ES cells.	1-4 days
Targeting Vector Construction	Clone necessary DNA fragments into the targeting vector. Validate construction through sequencing and restriction digest.	6-8 weeks
Targeting in ES Cells	Electroporate TurboKnockout® ES cells, screen for targeted clones via PCR, and confirm by Southern blot and karyotyping.	8-14 weeks
Mouse Production	Introduce targeted ES cells into host embryos, transfer to surrogate mothers, and genotype founders. Breeding to F1 generation.	18-20 weeks

Note:

Turnaround times exclude institutional approval and shipping durations. For specific strain requests or larger knockin fragments, please inquire about custom solutions and pricing.

Targeted Gene Editing Technology for Knockin Mice at Any Locus

Discover Cyagen’s Targeted Gene Editing technology for large-fragment and conditional knockin mouse models. This versatile approach allows precise gene editing at any genetic locus, making it ideal for diverse research applications including disease modeling and functional studies.

Stage	Description	Turnaround Time
Strategy Design	Develop a nuclease-mediated strategy tailored to your gene of interest, including gRNA and donor vector design.	1-4 days
Vector Construction	Construct the knockin vector based on client-approved strategy. Where needed, efficacy tests in cell culture can be conducted.	6-8 weeks
Targeted Gene Editing Injection	Co-inject ribonucleoprotein (RNP) and the donor vector into fertilized mouse eggs, and implant to obtain founders.	10-14 weeks
Founder Screening	Screen pups by PCR to identify those with successful knockin or floxed modifications. Where needed, Sanger sequencing can be conducted.	10-14 weeks
Breeding Founders	Breed the founders to wildtype mice and genotype their offspring to ensure transmission of the knockin allele.	12-16 weeks

Note:

Standard services cover knockin fragments up to 8 kb. Larger fragments may incur additional costs and time.
All projects are conducted in the C57BL/6 strain by default, with options for other strains and rat models.

Targeted Gene Editing Technology for Knockin Mice at ROSA26/H11 Locus

Delve into Cyagen’s Targeted Gene Editing technology optimized for large-fragment knockin at the ROSA26/H11 locus, known for its reliability as a "safe harbor" site that ensures stable gene expression without disrupting endogenous genes. This method is ideal for researchers aiming to achieve consistent and ubiquitous gene expression across various applications.

Stage	Description	Turnaround Time
Strategy Design	Develop a nuclease-mediated strategy tailored to your gene of interest, including gRNA and donor vector design.	1-4 days
Vector Construction	Construct the ROSA26/H11 knockin vector based on client-approved strategy. Where needed, efficacy tests in cell culture can be conducted.	4-7 weeks
Targeted Gene Editing Injection	Co-inject ribonucleoprotein (RNP) and the donor vector into fertilized mouse eggs at the ROSA26/H11 locus, followed by embryo implantation to obtain founders.	10-14 weeks
Founder Screening	Screen pups by PCR to identify those with successful knockin at ROSA26/H11 locus. Where needed, Sanger sequencing can be conducted.	10-14 weeks
Breeding Founders	Breed the founders to wildtype mice and genotype their offspring to ensure transmission of the knockin allele.	12-16 weeks

Note:

Services include knockin fragment insertion up to 8 kb. Larger fragments may involve additional costs and time.
Standard procedures are performed in the C57BL/6 strain, with options for other strains upon request.

Targeted Gene Editing Technology for Point Mutation Mice

Explore Cyagen’s Targeted Gene Editing technology tailored for point mutation knockin mice, ideal for studying genetic disorders, rare diseases, and functional genomics. This precise gene editing technique allows for the introduction of specific nucleotide changes that model human genetic variations accurately.

Stage	Description	Turnaround Time
Strategy Design	Develop a nuclease-mediated strategy tailored to your gene of interest, including gRNA and donor oligo design.	1-4 days
Targeted Gene Editing Injection	Co-inject ribonucleoprotein (RNP) and the donor oligo into fertilized mouse eggs, and implant to obtain founders.	10-12 weeks
Founder Screening	Screen pups by PCR and Sanger sequencing to identify those with the correct point mutation knockin.
Off-Target Analysis	Analyze potential off-target effects to ensure specificity of the gene edit.

Note:

Turnaround times do not include institutional approval processes or shipping.
Services focus on knockin modifications in the C57BL/6 strain, with options for other strains.

FAQs

What Customers Say About Cyagen

Violet Shimmer

Stanford University

The service provided to us by Cyagen is now in press at Nature as an article.

Scarlett Rouge

Seattle Children’s Hospital

We are very pleased with the state-of-the-art professional transgenic services provided by Cyagen for our study published recently in Nature. We continue to use Cyagen’s transgenic services as it allows us to do better and more efficient research with transgenic mice.

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