Standard AAV Packaging

Cyagen offers high-purity, high-titer AAV vectors for gene therapy and in vivo research. Our optimized three-plasmid system ensures high infection efficiency, low endotoxin levels, and broad serotype compatibility.

Fast & Reliable Delivery

Standard AAV projects ship in as fast as 2 weeks with rigorous QC testing.

Broad Serotype Selection

Offers multiple serotypes for different tissues and applications.

High Purity & Titer

Achieves robust gene expression in dividing and non-dividing cells.

Overview

Workflow

FAQs

Overview

High-Quality AAV Packaging for Gene Therapy

Adeno-associated virus (AAV) vectors are the most widely used viral vectors for gene therapy due to their low immunogenicity, stable expression, and ability to transduce both dividing and non-dividing cells.

Cyagen offers a reliable AAV packaging service with guaranteed high purity, titer, and quality control. Our production process ensures that researchers can receive ready-to-use viral vectors optimized for in vivo and in vitro applications.

Enhancing AAV Gene Delivery: A Case Study in Optimized Serotype Selection
See how customized AAV serotype selection improved gene delivery in a preclinical study, addressing vector tropism, transduction efficiency, and immune response challenges.

Explore Ready-to-Use Mouse Models

Discover over 18,000 validated mouse strains—including knockout, conditional knockout, and humanized models—covering 20+ research areas such as oncology, neurology, and metabolism. All models are supported by detailed genotype data and guaranteed quality, helping you fast-track discovery with confidence.

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Workflow

Workflow and Delivery

Cyagen’s AAV packaging service ensures high-purity, high-titer virus production with customizable options tailored for gene therapy and biomedical research. Below is an overview of our streamlined workflow, service specifications, and available serotypes.

Service Comparison & Deliverables

We offer different grades of lentiviral packaging services tailored to various applications, ensuring the highest level of performance and biosafety compliance.

AAV Type	Production Scale	Virus Amount	Titer	Turnaround	QC & Deliverables
Overexpression	Pilot	≥1×10¹² GC	≥1×10¹² GC/mL	5-6 weeks	- Purified AAV particles (selected serotype) - Complimentary control virus - qPCR-based titer quantification - SDS-PAGE & Western blot validation - Endotoxin testing (<10 EU/mL) - Purity validation (≥95%)
Overexpression	Medium	≥5×10¹² GC	≥1×10¹³ GC/mL	5-6 weeks
RNA Interference	Pilot (3+1)	≥1×10¹² GC	≥1×10¹² GC/mL	5-6 weeks
RNA Interference	Medium (3+1)	≥5×10¹² GC	≥1×10¹³ GC/mL	5-6 weeks
Knockout	Pilot	≥1×10¹² GC	≥1×10¹² GC/mL	5-6 weeks
Knockout	Medium	≥5×10¹² GC	≥1×10¹³ GC/mL	5-6 weeks

Workflow & Timeline

Stage	Description	Estimated Time
Plasmid Preparation	Three-plasmid co-transfection system design & optimization with AI-assisted sequence validation.	1 week
AAV Production	293T cell transfection and virus harvesting.	2-3 weeks
Purification	Gradient ultracentrifugation for high-purity AAVs.	1-2 weeks
Quality Control & Validation	Titer quantification, purity assessment, sterility testing.	1-2 weeks
Final Packaging & Delivery	Sterile filtration, cryopreservation, documentation preparation.	1 week

Notes: Total Turnaround: 5-6 weeks (depending on vector type & serotype selection).

FAQs

What Customers Say About Cyagen

Violet Shimmer

Stanford University

The service provided to us by Cyagen is now in press at Nature as an article.

Scarlett Rouge

Seattle Children’s Hospital

We are very pleased with the state-of-the-art professional transgenic services provided by Cyagen for our study published recently in Nature. We continue to use Cyagen’s transgenic services as it allows us to do better and more efficient research with transgenic mice.

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