Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Induced Pluripotent Stem Cells (iPSCs)
Cyagen’s iPSC services provide high-efficiency reprogramming, precise gene editing, and optimized differentiation for disease modeling, drug discovery, and regenerative medicine.
Optimized Differentiation
≥95% marker expression for high-purity cells.
Advanced Gene Editing
Optimized HDR up to 50%, KO efficiency 90%.
Efficient Reprogramming
Non-integrating method with 99% success rate.
Overview
Workflow
FAQs
Overview
Comprehensive iPSC Solutions
Cyagen provides comprehensive iPSC solutions designed to support various research applications, including iPSC reprogramming, gene editing, and directed differentiation.
— For iPSC reprogramming, we generate high-quality iPSC lines from somatic cells using non-integrating methods, ensuring genetic stability. Each iPSC line undergoes pluripotency and karyotype validation to meet rigorous research standards.
— Our iPSC gene editing services enable precise genetic modifications, including knockout, knock-in, and point mutations. With our optimized HDR technology, we achieve up to 50% efficiency, enhancing the accuracy and success rate of genome edits.
— For iPSC differentiation, we provide specialized cell models, including neurons, blood cells, and liver organoids, tailored for disease research and drug development. Each model is validated through immunofluorescence and qPCR to ensure functionality and reproducibility.
Explore Ready-to-Use Mouse Models
Discover over 18,000 validated mouse strains—including knockout, conditional knockout, and humanized models—covering 20+ research areas such as oncology, neurology, and metabolism. All models are supported by detailed genotype data and guaranteed quality, helping you fast-track discovery with confidence.
You Might Also Be Interested In
Knockout Cell Lines Library
Browse validated human KO cell lines by gene or cell type
Tumor Cell Lines Library
Access characterized human tumor lines by cancer type or genetic status.
Cell Immunotherapy CRO Platform
CAR-T/NK development with custom vectors, models, and in vivo validation
Targeted Gene Editing
AI-enhanced HDR technology for precise in vivo genome engineering
MouseAtlas Model Library
Search and access curated genetically engineered mouse strains
AAV Vectors Library
Search and order AAVs by serotype, gene, or function
Workflow
Workflow and Delivery
Cyagen provides a streamlined, high-efficiency workflow for iPSC development, from reprogramming and gene editing to directed differentiation. Our optimized processes ensure high success rates, rigorous quality control, and fast turnaround times to support your research needs.
iPSC Reprogramming
iPSC Gene Editing
iPSC Directed Differentiation
iPSC Reprogramming
iPSC Reprogramming
Cyagen offers high-quality episomal-based iPSC reprogramming, ensuring genetic stability and compatibility with downstream applications.
Deliverables & QC
Project Deliverables QC Measures Turnaround
iPSC Reprogramming 1 iPSC clone (2 vials, 1×10⁶ cells/vial) Flow cytometry, karyotyping, differentiation assay (optional) 12+ weeks
Workflow & Timeline
Stage Description Timeframe
PBMC or Fibroblast expansion 1. Mycoplasma and sterility testing.
2. Cell proliferation assessment.
1-2 weeks
iPSC Clone Formation Sendai virus transduction, formation of selectable iPSC clones 2-3 weeks
iPSC Purification & Expansion Removal of exogenous gene residues, stabilization of iPSC state 7-8 weeks
iPSC Characterization STR, karyotype analysis, flow cytometry,mycoplasma and sterility testing, immunofluorescence 2-3 weeks
In Vitro Tri-lineage Differentiation (optional) In viotro pluripotency assay for further validation 2-3 weeks
Teratoma Formation Assay (Optional) In vivo pluripotency assay for further validation 8-12 weeks
Note:
  • Standard Reprogramming: 12–16 weeks
  • With Additional Validation Assays (optional): Up to 31 weeks
iPSC Gene Editing
iPSC Gene Editing
Our optimized gene editing platform enables knockout, knock-in, and point mutation in iPSCs with high precision.
Deliverables & QC
Project Deliverables QC Measures Timeframe
Knockout (KO) 1 monoclonal homozygous line, 2 vials PCR, sequencing, immunofluorescence 8+ weeks
Point Mutation (PM) 1 monoclonal homozygous mutant line PCR, sequencing, immunofluorescence 12+ weeks
Knock-in (KI) 1 monoclonal heterozygous knock-in line (homozygous optional) PCR, , sequencing, immunofluorescence 12+ weeks
Workflow & Timeline
Stage Description Timeframe
Cell Expansion & QC 1. Mycoplasma and sterility testing
2. Cell proliferation assessment
3. Primer design for sequencing the target region
1–2 weeks
Guide & Donor Design Design and synthesis of genomic targeting guides and (if applicable) donor templates for homology-directed repair (HDR) 2–6 weeks
Electroporation & Clone Selection Delivery of gene editing reagents into iPSCs Single-cell clone expansion, PCR screening, and sequencing validation 4–8 weeks
Cryopreservation & Final QC 1. Sequence confirmation of edited site
2. Mycoplasma and sterility testing
3. Viability testing
4. Immunofluorescence for pluripotency markers
1–2 weeks
Note:Total Estimated Time: 8-18+ weeks
iPSC Directed Differentiation
iPSC Directed Differentiation
Cyagen offers custom iPSC differentiation services for generating functionally validated cell types.
Deliverables & QC
Project Deliverables QC Measures Timeframe
Motor Neuron Progenitors 3 vials (3 × 10⁶ cells/vial)
*Includes mature culture medium for differentiation into motor neurons*
Immunofluorescence (MNP Oligo2/MNs CHAT), mycoplasma and sterility testing 6+ weeks
Hematopoietic Progenitor Cells (HPCs) 2 vials (1×10⁶ cells/vial) Flow cytometry, mycoplasma and sterility testing 4+ weeks
Neural progenitors cells (NPCs) 2 vials (1×10⁶ cells/vial) Immunofluorescence (SOX2/PAX6/Nestin), mycoplasma and sterility testing 3+ weeks
Retinal Pigment Epithelium (RPE) 3 vials (3 × 10⁶ cells/vial) Immunofluorescence (MiTF/ZO-1), mycoplasma and sterility testing 8+ weeks
Workflow & Timeline
Stage Description Timeframe
iPSC Cell Expansion & QC 1. Mycoplasma and sterility testing
2. Cell expansion
1-2 weeks
Differentiation Differentiation initiation 1-6weeks
Maturation & QC Differentiated cells QC 1-2 weeks
Note:Total Estimated Time: 3-8+ weeks
FAQs
Frequently Asked Questions (FAQs)
How do iPSCs differ from ESCs?
iPSCs avoid ethical concerns while offering similar differentiation potential.
Can iPSCs be used for personalized medicine?
Yes, patient-derived iPSCs enable autologous cell therapy research.
How do you ensure iPSC genetic stability?
We use episomal vectors and conduct karyotype & STR analysis.
What gene editing strategies do you offer?
We support knockout, knock-in, point mutations, and stable expression.
How do you validate differentiation outcomes?
Immunofluorescence, flow cytometry, and qPCR are used for confirmation.
What Customers Say About Cyagen
Violet Shimmer
Stanford University
The service provided to us by Cyagen is now in press at Nature as an article.
Scarlett Rouge
Seattle Children’s Hospital
We are very pleased with the state-of-the-art professional transgenic services provided by Cyagen for our study published recently in Nature. We continue to use Cyagen’s transgenic services as it allows us to do better and more efficient research with transgenic mice.
Request a Custom Cell Line Consultation
Tell us about your cell line project needs. Our specialists are ready to support your research with tailored solutions.
Inquiry Details
Main Area of Research
Service(s) of Interest
Gene of Interest
Project Details
Contact Information
Full Name
Email
Phone Number
Organization
Job Role
Country
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our  Privacy Policy  for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest