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Cryopreservation & Recovery
Preserve your genetically modified lines through sperm or embryo cryopreservation—and recover live animals within weeks when needed.
Storage & Guarantee
Samples stored at Cyagen are covered by recovery success guarantees.
On-Demand Recovery
Get live animals within 8–12 weeks from preserved stock.
Reliable Genetic Backup
Protect key strains against loss, contamination, or facility failure.
Overview
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Overview
Secure Your Strains with Reliable Cryopreservation
Cyagen offers comprehensive embryo and sperm cryopreservation services to safeguard your valuable strains and reduce the need for continuous breeding. Once stored at our AAALAC-accredited, OLAW-assured facility, your samples can be revived within 8–12 weeks upon request. Our cryopreservation services are ideal for rarely used models, disaster preparedness, or strain transport reduction.

Service Types, Descriptions, Requirements, and Deliverables:

1. Embryo Cryopreservation
Description: 2-cell embryos are frozen and stored in liquid nitrogen.
Requirements: 4–8 males aged 6–8 weeks.
Deliverables: At least 200 embryos per line.

2. Sperm Cryopreservation
Description: Motile sperm collected and cryopreserved from healthy males.
Requirements: At least 2 males aged 8–16 weeks.
Deliverables: 4–5 vials per line.

3. Embryo Cryorecovery
Description: Thawed embryos implanted into pseudopregnant mice.
Requirements: Cryostock stored at Cyagen.
Deliverables: At least 2 heterozygous animals within 8–12 weeks.

4. Sperm Cryorecovery
Description: IVF followed by embryo transfer using thawed sperm.
Requirements: Cryostock stored at Cyagen.
Deliverables: At least 2 heterozygous animals within 8–12 weeks.
Explore Ready-to-Use Mouse Models
Discover over 18,000 validated mouse strains—including knockout, conditional knockout, and humanized models—covering 20+ research areas such as oncology, neurology, and metabolism. All models are supported by detailed genotype data and guaranteed quality, helping you fast-track discovery with confidence.
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MouseAtlas Model Library
Search and access curated genetically engineered mouse strains
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Scalable colony expansion with full genotyping support
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Full-spectrum analysis for rodents model
Turboknockout® Gene Editing
Fast-track your research with our efficient gene targeting service.
Targeted Gene Editing
AI-enhanced HDR technology for precise in vivo genome engineering
Knockout Cell Lines Library
Browse validated human KO cell lines by gene or cell type
Workflow
Workflow and Delivery
Understanding the timeline and key steps of cryopreservation and recovery helps you plan experiments with confidence. Whether you’re storing strains for future use or preparing for a rapid recovery, our standardized process ensures efficiency, genetic fidelity, and predictable turnaround times.
Cryopreservation Path
Recovery Path
FAQs
Frequently Asked Questions (FAQs)
How long can cryopreserved samples stay viable?
Embryos and sperm remain viable for 10+ years in liquid nitrogen without quality loss.
How fast can animals be recovered?
Recovery typically takes 8–12 weeks, depending on stock type and availability.
Is this service useful for rare-use or backup strains?
Absolutely. It saves space, cost, and protects your colony in case of facility failure.
Do you guarantee live animal delivery?
Yes—for cryostocks stored at Cyagen, we guarantee delivery of ≥2 heterozygous animals.
Can I cryopreserve strains developed elsewhere?
Yes, we accept external lines. Guarantees may vary—please contact us for eligibility.
What Customers Say About Cyagen
Violet Shimmer
Stanford University
The service provided to us by Cyagen is now in press at Nature as an article.
Scarlett Rouge
Seattle Children’s Hospital
We are very pleased with the state-of-the-art professional transgenic services provided by Cyagen for our study published recently in Nature. We continue to use Cyagen’s transgenic services as it allows us to do better and more efficient research with transgenic mice.
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