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Lentivirus Packaging
High-efficiency lentiviral vector production for gene delivery, CAR-T cell therapy, and cell line construction. Cyagen provides scalable solutions with strict quality control to ensure high-titer, high-purity lentivirus for your research.
Reliable & Ready-to-Use
Validated for stable gene expression and hard-to-infect cells.
Scalable High-Titer Production
Purified lentivirus (≤1×10⁹ TU/mL) for in vivo & in vitro use.
High Biosafety Standards
3rd-gen lentiviral system for safety & high efficiency.
Overview
Workflow
FAQs
Overview
Comprehensive Lentivirus Packaging for Reliable Gene Delivery
Lentiviral vectors provide an effective method for stable gene delivery, widely used in cell line engineering, gene therapy, and CAR-T applications. Cyagen offers a comprehensive lentivirus packaging service, covering overexpression, interference, and knockout applications. Our production system ensures high-quality, high-titer lentivirus with stringent quality control for both research and therapeutic applications.
With extensive experience in viral vector production, we provide scalable solutions that include purified, high-titer lentivirus suitable for both in vitro and in vivo applications. Our flexible service options cater to different experimental needs, allowing for custom vector construction and packaging strategies.
Explore Ready-to-Use Mouse Models
Discover over 18,000 validated mouse strains—including knockout, conditional knockout, and humanized models—covering 20+ research areas such as oncology, neurology, and metabolism. All models are supported by detailed genotype data and guaranteed quality, helping you fast-track discovery with confidence.
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Search and order AAVs by serotype, gene, or function
MouseAtlas Model Library
Search and access curated genetically engineered mouse strains
Cell Immunotherapy CRO Platform
CAR-T/NK development with custom vectors, models, and in vivo validation
Gene Therapy CRO Platform
AI-guided AAV design and full-spectrum preclinical validation
Workflow
Workflow and Delivery
Cyagen’s optimized virus packaging workflow ensures reliable, high-titer lentivirus production with rapid turnaround times. Our comprehensive process includes stringent quality control at every stage, delivering lentiviral vectors suitable for a wide range of research and therapeutic applications.
Service Comparison
We offer different grades of lentiviral packaging services tailored to various applications, ensuring the highest level of performance and biosafety compliance.
Type Titer Quality Control Turnaround Application
Standard Lentivirus ≥1×10⁸ TU/mL qPCR, flow cytometry 2 weeks Cell transduction
Purified Lentivirus ≥1×10⁸ TU/mL qPCR, ELISA, endotoxin test 2 weeks In vivo injection
High-Titer Lentivirus ≥1×10⁹ TU/mL qPCR, ELISA, flow cytometry 2 weeks High-efficiency gene transfer
Workflow & Timeline
Our lentivirus production follows a standardized, high-efficiency workflow to ensure consistency and reproducibility.
Stage Description Timeframe
Plasmid Preparation Selection and preparation of transfer, envelope, and packaging plasmids. 1 week
Transfection & Virus Production Transfection of 293T cells using the 3rd-generation lentiviral system. 2-3 weeks
Lentivirus Harvesting & Purification Collection and processing of standard or purified lentivirus. 1 week
Titer Quantification & QC qPCR, flow cytometry, functional validation for high accuracy. 1-2 weeks
Final Packaging & Delivery Mycoplasma and sterility testing (if required). 1 week
Notes: Total Turnaround: 5-8 weeks (varies based on purification and titer requirements).
FAQs
Frequently Asked Questions (FAQs)
Can I request a specific lentiviral vector?
Yes, we offer fully customizable lentivirus vector construction.
How do I determine the MOI (Multiplicity of Infection)?
We provide detailed viral titer information and assist with MOI calculations.
What quality control measures are in place?
qPCR, flow cytometry, and functional validation are standard. Endotoxin testing is available for purified lentivirus.
Do you offer large-scale lentivirus production?
Yes, scalable production is available for preclinical and translational research.
What biosafety level is required for lentivirus work?
Cyagen’s lentivirus packaging uses 3rd-generation lentiviral systems, classified as BSL-2.
What Customers Say About Cyagen
Violet Shimmer
Stanford University
The service provided to us by Cyagen is now in press at Nature as an article.
Scarlett Rouge
Seattle Children’s Hospital
We are very pleased with the state-of-the-art professional transgenic services provided by Cyagen for our study published recently in Nature. We continue to use Cyagen’s transgenic services as it allows us to do better and more efficient research with transgenic mice.
Request a Virus Packaging Service Consultation
Get expert guidance for your viral vector production needs. Submit your project details and we'll assist you promptly.
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