Can Cre mice be used for both loss-of-function and gain-of-function studies?

Yes, Cre mice can be used for both loss-of-function (knockout) and gain-of-function (overexpression) studies. By choosing the right mouse model and targeting specific genes, you can explore gene functions in a variety of experimental settings.

What factors influence the efficiency of Cre recombination in mice?

Cre recombination efficiency can be influenced by factors like tissue type, Cre expression levels, and the presence of specific promoter sequences. Our team offers expert advice on optimizing these variables to ensure consistent and reliable results.

How far apart can the loxP sites be for effective gene recombination?

The distance between loxP sites can vary, but for optimal recombination efficiency, they should be relatively close. The ideal distance depends on the specific gene and tissue type you're working with, and we can provide guidance on the best configuration for your research.

What’s the best way to start breeding Cre Lox mice for my research?

Starting with Cre Lox breeding requires careful planning of mouse lines. You'll need to select appropriate Cre and floxed lines and follow strategies to ensure proper genetic recombination. We can assist you with protocols and advice on successful breeding.

How does “Floxing” differ from Cre/loxP gene knockout?

"Floxing" refers to the process of inserting loxP sites around a gene, while Cre/loxP knockout involves using the Cre enzyme to remove the gene between those sites. This allows for targeted and conditional gene modification in specific tissues or stages.

Do all Cre mice models result in gene knockout?

Not all Cre mice are designed for gene knockout. While many models use Cre to delete floxed genes, some are designed for conditional gene activation or other modifications. It’s important to choose the right Cre model based on your research goals.

Cre Mouse Models for Targeted Gene Research

Cre Mouse Models for Gene Research

Control gene expression in time and space. The Cre/loxP system lets you modify genes in specific tissues and at defined times, by utilizing the Cre/loxP recombination technique, you can create genetically modified mice that allow for targeted gene modification. Every experiment advances. Every modification matters.

Research-Ready Delivery

Expedited shipping within X hours. Comprehensive health reports included. Direct access to technical support.

Validated Performance

Every line validated through PCR and sequencing. Full breeding documentation provided. Guaranteed germline transmission.

Site-Specific Control

Target genes precisely. Control expression timing. Execute conditional knockouts without developmental interference.

Overview

Product

FAQs

Overview

Versatile Cre Mouse Lines: Your Gateway to Conditional Genetics

From regular Cre to inducible variants, our validated mouse lines drive tissue-specific gene manipulation across cardiology, neuroscience, and ophthalmology research. Each line comes with comprehensive expression data and breeding validation, empowering your conditional knockout and knockin studies with proven genetic precision.

Explore Ready-to-Use Mouse Models

Discover over 18,000 validated mouse strains—including knockout, conditional knockout, and humanized models—covering 20+ research areas such as oncology, neurology, and metabolism. All models are supported by detailed genotype data and guaranteed quality, helping you fast-track discovery with confidence.

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Product

Catalog Number	Name	Base Strain	Expressing Tissues/Cells
I001062	Adgrg2-P2A-Cre	C57BL/6NCya	Testicles
I001169	Adgre1-P2A-CreERT2	C57BL/6JCya	Macrophages
C001743	Adgrl2-3xGGGGS-mCherry	C57BL/6JCya	Various tissues, including the brain and heart, and Adgrl2-positive cells
C001529	Adipoq-iCre	C57BL/6JCya	Adipocytes
C001558	Agrp-IRES-CreERT2-P2A-tdTomato	C57BL/6JCya	--
I001165	Aqp5-P2A-CreERT2	C57BL/6JCya	Alveolar type I cells, salivary gland alveolar cells, and gastrointestinal stem cells
I001079	Aire-P2A-CreERT2	C57BL/6JCya	Medullary Thymic Epithelial Cells (mTECs)
I001112	Aldh1l1-P2A-Cre	C57BL/6JCya	Astrocytes
C001746	Alpl-NLS-mScarlet	C57BL/6JCya	Liver, kidney, bone tissue, placenta, intestine, and Alpl-positive cells
C001648	Ace2-P2A-CreERT2	C57BL/6JCya	Kidney, lung, brain, small intestine, large intestine and Ace2-positive cells

FAQs

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