What types of diseases or conditions are studied using knockout mice?

Knockout mice have been used in cancer, cardiovascular disease, neurodegenerative disorder, and immunology studies. Some examples include studying the role of tumor suppressor genes in cancer, investigating the molecular mechanisms of Alzheimer's disease, and exploring the immune response to viral infections.

Custom Knockout Mouse Models for Disease Research

Knockout Mice

With the capability to modify mouse genomes, genetically engineered knockout mice have become essential for modeling human diseases. At Cyagen, we support clients globally with diverse knockout mouse models, offering study-ready cohorts tailored to their research requirements.

Cost-Effective Solutions

High-quality gene targeting at unbeatable prices.

Swift Turnaround Time

Get your gene targeting mouse models faster.

Guaranteed Results

Get animals or your money back.

Overview

Workflow

FAQs

Overview

Comprehensive Gene Targeting Solutions

We provide comprehensive solutions for your gene targeting mouse model requirements, offering both conventional knockout and conditional knockout services. Whether you need assistance with the entire targeting project, from strategy design to mutant animal delivery, or prefer to outsource specific phases, we've got you covered. With the shortest turnaround time in the industry and unbeatable prices, trust us for efficient and cost-effective solutions tailored to your needs.

Our knockout mouse models offer invaluable resources for investigating gene function and modeling human diseases. Whether you require conventional knockout for permanent gene disruption or conditional knockout for tissue-specific targeting, we have the solution to meet your research needs.

Explore Ready-to-Use Mouse Models

Discover over 18,000 validated mouse strains—including knockout, conditional knockout, and humanized models—covering 20+ research areas such as oncology, neurology, and metabolism. All models are supported by detailed genotype data and guaranteed quality, helping you fast-track discovery with confidence.

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Turboknockout^® Gene Editing

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Targeted Gene Editing

AI-enhanced HDR technology for precise in vivo genome engineering

Rodent Breeding

Scalable colony expansion with full genotyping support

Cryopreservation & Recovery

Preserve and revive rodent strains on demand

Rodent Phenotyping

Full-spectrum analysis for rodents model

Workflow

Workflow and Delivery

Our streamlined process ensures efficient generation and delivery of knockout mouse models to meet your research timelines. Choose from our two advanced gene editing technologies, TurboKnockout^® and Targeted Gene Editing, each offering unique advantages for precise gene targeting.

TurboKnockout^® Technology

Explore our efficient TurboKnockout^® process designed for precise and timely delivery of knockout mouse models.

Stage	Description	Turnaround Time
Design	Collaborate on gene targeting strategy analysis and approval.	1-4 days
Vector Construction	Construction and validation of targeting vector through sequencing and restriction digest.	6-8 weeks
Targeting in ES Cells	Electroporation into TurboKnockout^® ES cells (C57BL/6 or BALB/c), PCR screening, Southern blot, karyotyping.	8-14 weeks
Mouse Production	Introduction of targeted ES cells into host embryos, optional breeding to F1, genotype verification.	18-20 weeks
Validation & Delivery	Final phenotype and genotype validation of mice, delivery of study-ready cohorts.	Depends on breeding choice

Note:
Pricing details are available upon inquiry. Optional services include extended breeding and specific strain requests. Turnaround times exclude institutional approval and shipping durations.

Targeted Gene Editing Technology

Leverage our cutting-edge Targeted Gene Editing technology for rapid and precise knockout mouse generation, designed to meet stringent research specifications.

Stage	Description	Turnaround Time
Design	Provide the gene name for knockout; we design a nuclease-mediated strategy optimizing on-target activity and minimizing off-targets.	1-4 days
Injection	Inject nuclease mRNA into fertilized mouse eggs, followed by implantation into surrogate mothers to produce founders.	10-14 weeks
Founder Screening	Screen pups by PCR and sequencing to identify those with desired mutations; frameshift or critical exon deletions indicate knockouts.	10-14 weeks
Breeding Founders	Optionally breed up to three founders to wildtype mice to obtain F1 mice carrying the knockout allele.	12-16 weeks
Validation & Delivery	Validate knockout mice through genotyping and off-target analysis, and deliver study-ready cohorts.	Depends on breeding

Note:

Pricing: Details are available upon inquiry for each service stage.
Strain Information: Services are primarily offered in the C57BL/6 strain, with options for other strains and rat models.
Turnaround Time Exclusions: Excludes time for obtaining host institution's approval for mouse importation and transit time during shipping.

FAQs

Citation Database

Molecular Therapy: Methods & Clinical Development, March, 2025

Intracranial AAV administration dose-dependently recruits B cells to inhibit the AAV redosing

【Other】

Gut, February, 2025

E-twenty-six-specific sequence variant 5 (ETV5) facilitates hepatocellular carcinoma progression and metastasis through enhancing polymorphonuclear myeloid-derived suppressor cell (PMN-MDSC)-mediated immunosuppression

【Other】

Cell Death & Disease, February, 2025

Mcm5 mutation leads to silencing of Stat1-bcl2 which accelerating apoptosis of immature T lymphocytes with DNA damage

【Other】

Molecular Therapy, February, 2025

Single-cell data-driven design of armed oncolytic virus to boost cooperative innate-adaptive immunity against cancer

【Other】

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