Mice are the most widely used animal model for studying gene function and understanding human health. The high degree of biological and genetic similarity that mice share with humans have made mouse models a powerful tool to study the mechanisms of human disease and develop new drugs.
Advantages of Mouse Models
- Genetic similarity: Mice develop many of the same diseases as humans, often due to the same genetic and pathophysiological factors.
- Modifiable genome: Genomic engineering technologies enable humanized mice, which can mimic nearly any human disease or genetic condition.
- Accelerated lifespan: 2-year lifespan (1-year old mouse ≈ 37 human years of age); Quickly progress through generations, observing age-related diseases in less time.
- Quick breeding: 20-21 days gestation, potential for large litter size (dependent upon background strain)
- Well-characterized: Numerous genetically defined background strains of inbred mice provide stable phenotypes.
- Economical: Small size reduces upkeep costs and lowers facility fees.
- Studies of gene function & involvement in disease pathogenesis
- In vivo target discovery and confirmation
- Mechanism of action (MOA) study
- Verifying compound specificity & detecting potential off-target effects
Cyagen’s Custom Mouse Model Generation Services
| Genetic Modification Technology |
Advantages |
Mouse Model
Generation Capabilities |
| TurboKnockout® Gene Targeting Mice |
- No patent risk: Technology of choice for drug development projects
- Success rate: 100% guaranteed germline transmission (GLT)
- Turnaround time: Founders as fast as 6-8 months
- Size limitations: Knockin (KI) fragment size up to 300 kb
- loxP restrictions: No size limitations for your floxed region
- 100% Money-Back Guarantee
|
|
| Donor Strains: C57BL/6, BALB/c |
| CRISPR-mediated |
- Short timeline: F0 animals as fast as 3 months
- Large-fragment modifications: Success with LFKI up to 15kb and 400kb knockout.
- Economical: Rapid production of models at a competitive price
- Targeted: Express your gene of interest accurately and precisely.
- High efficiency: Our unique mouse egg fertilization process improves the efficiency of homology-directed repair (HDR).
|
|
| Donor Strains: C57BL/6, FVB – other strains upon request. |
| Transgenic |
Cyagen new PiggyBac transgenic mouse service can deliver single-copy transgenic mice in as fast as 3 months.
- Single-copy integration: Avoids potential gene silencing from multiple copies
- Defined region of integration: No loss of transgene sequence (TTAA, transcription unit)
- Reliability: More consistent expression pattern compared to plasmid based transgenics
- Economical: Cost and turnaround time comparable to plasmid based transgenics
|
|
| Donor Strains: C57BL/6, FVB – other strains upon request. |
