Regular Transgenesis
Regular transgenesis is a widely used method for generating rodent models with strong transgene expression. Through pronuclear injection, it enables in vivo studies of gene overexpression and regulatory elements.
Reliable and Cost-Effective
A proven, economical choice ideal for exploratory research.
Flexible Construct Options
Supports plasmid and BAC vectors for small to large (>100 kb) transgenes.
Fast and High Expression
Generate founders in 2–5 months with strong, multi-tissue transgene expression.
Technology
Applications
FAQs
How Regular Transgenesis Works
In regular transgenesis, linearized DNA constructs are microinjected
into the pronucleus of fertilized mouse or rat embryos. These constructs
integrate randomly into the host genome—typically in multiple copies—and
result in hemizygous expression of the transgene. The founders are
screened for integration and expression, then bred for colony expansion
or experimental use.
Integration Characteristics
- Random genomic integration
- Multi-copy insertion per site
- Variable expression patterns among founders
Expression Outcomes
- Often strong expression in early generations
- Potential for position-effect variegation due to integration site randomness
Citation Database
Molecular Therapy: Methods & Clinical Development, March, 2025
Intracranial AAV administration dose-dependently recruits B cells to inhibit the AAV redosing
【Other】
Gut, February, 2025
E-twenty-six-specific sequence variant 5 (ETV5) facilitates hepatocellular carcinoma progression and metastasis through enhancing polymorphonuclear myeloid-derived suppressor cell (PMN-MDSC)-mediated immunosuppression
【Other】
Cell Death & Disease, February, 2025
Mcm5 mutation leads to silencing of Stat1-bcl2 which accelerating apoptosis of immature T lymphocytes with DNA damage
【Other】
Molecular Therapy, February, 2025
Single-cell data-driven design of armed oncolytic virus to boost cooperative innate-adaptive immunity against cancer
【Other】
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