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Regular Transgenesis
Regular transgenesis is a widely used method for generating rodent models with strong transgene expression. Through pronuclear injection, it enables in vivo studies of gene overexpression and regulatory elements.
Reliable and Cost-Effective
A proven, economical choice ideal for exploratory research.
Flexible Construct Options
Supports plasmid and BAC vectors for small to large (>100 kb) transgenes.
Fast and High Expression
Generate founders in 2–5 months with strong, multi-tissue transgene expression.
Technology
Applications
FAQs
Technology
How Regular Transgenesis Works
In regular transgenesis, linearized DNA constructs are microinjected into the pronucleus of fertilized mouse or rat embryos. These constructs integrate randomly into the host genome—typically in multiple copies—and result in hemizygous expression of the transgene. The founders are screened for integration and expression, then bred for colony expansion or experimental use.
Integration Characteristics
  • Random genomic integration
  • Multi-copy insertion per site
  • Variable expression patterns among founders
Expression Outcomes
  • Often strong expression in early generations
  • Potential for position-effect variegation due to integration site randomness
Applications
Applications of Regular Transgenesis
This method is ideal for applications that require:
·Gene overexpression or dominant-negative constructs
·Tissue-specific expression using conditional promoters
·Large DNA insertions via BAC vectors

Explore Our Models & Services
Explore related services that expand on Regular Transgenesis for more precise or complex genetic modifications.
Transgenic Mice
Mice carrying foreign genes to explore gene overexpression and promoter activity.
Transgenic Rats
Rats engineered to express exogenous genes for disease and pathway research.
PiggyBac Transgenesis
Single-copy insertion system for stable, reproducible gene expression
BAC Transgenesis
Large-fragment delivery for accurate, native-like gene expression
BAC Modification
Custom genomic edits with full validation and transgenic support
MouseAtlas Model Library
Search and access curated genetically engineered mouse strains
FAQs
Frequently Asked Questions (FAQs)
What type of DNA constructs can be used for regular transgenesis?
We support both standard plasmid vectors and BAC constructs. Plasmids are suitable for smaller transgenes, while BACs accommodate genomic fragments up to 300 kb.
How many copies of the transgene are typically integrated?
Regular transgenesis often results in multiple tandem copies at a single integration site. This may lead to higher expression, but also carries risks of silencing or variable expression.
Is the integration site controlled or random?
Integration is random, which may lead to position effects or disruption of endogenous genes. Screening multiple founder lines is essential.
How long does the process take?
You can expect to receive founder animals in 2–5 months, depending on project complexity and strain availability.
Can regular transgenesis be used for precise gene knockin or conditional knockout?
No. For site-specific modifications, consider advanced technologies such as [Targeted Gene Editing] or [TurboKnockout®].
What Customers Say About Cyagen
Violet Shimmer
Stanford University
The service provided to us by Cyagen is now in press at Nature as an article.
Scarlett Rouge
Seattle Children’s Hospital
We are very pleased with the state-of-the-art professional transgenic services provided by Cyagen for our study published recently in Nature. We continue to use Cyagen’s transgenic services as it allows us to do better and more efficient research with transgenic mice.
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