Regular Transgenesis
Regular transgenesis is a widely used method for generating rodent models with strong transgene expression. Through pronuclear injection, it enables in vivo studies of gene overexpression and regulatory elements.
Reliable and Cost-Effective
A proven, economical choice ideal for exploratory research.
Flexible Construct Options
Supports plasmid and BAC vectors for small to large (>100 kb) transgenes.
Fast and High Expression
Generate founders in 2–5 months with strong, multi-tissue transgene expression.
Technology
Applications
FAQs
How Regular Transgenesis Works
In regular transgenesis, linearized DNA constructs are microinjected
into the pronucleus of fertilized mouse or rat embryos. These constructs
integrate randomly into the host genome—typically in multiple copies—and
result in hemizygous expression of the transgene. The founders are
screened for integration and expression, then bred for colony expansion
or experimental use.
Integration Characteristics
- Random genomic integration
- Multi-copy insertion per site
- Variable expression patterns among founders
Expression Outcomes
- Often strong expression in early generations
- Potential for position-effect variegation due to integration site randomness
What Customers Say About Cyagen
Violet Shimmer
Stanford University
The service provided to us by Cyagen is now in press at Nature as an article.
Scarlett Rouge
Seattle Children’s Hospital
We are very pleased with the state-of-the-art professional transgenic services provided by Cyagen for our study published recently in Nature. We continue to use Cyagen’s transgenic services as it allows us to do better and more efficient research with transgenic mice.
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