BAC Transgenesis
BAC (Bacterial Artificial Chromosome) transgenesis enables insertion of large genomic fragments—such as full genes with regulatory elements—into rodent genomes, ensuring faithful expression and supporting complex loci that are hard to replicate by other methods.
Suited for Complex Targets
Optimized for full-length human genes and loci with complex control regions.
Large Fragment Delivery
Deliver fragments up to 300 kb, preserving key regulatory elements for accurate gene expression.
Genetic Stability
Single-copy maintenance in bacteria ensures construct integrity and stable transmission.
Technology
Applications
FAQs
How BAC Transgenesis Works
BAC transgenesis involves injecting bacterial artificial chromosome
constructs into fertilized zygotes. These BACs carry large genomic
regions—including promoters, exons, introns, and enhancers—enabling
expression patterns that closely mimic native gene regulation. Because
the integration is random and multicopy, BAC models are typically
screened for expression and copy number.
Note: Compared to knockin strategies using homologous
recombination or gene editing, BAC transgenesis allows introduction of
entire genes without modifying endogenous loci—making it ideal for
exploratory or overexpression studies.
Citation Database
Molecular Therapy: Methods & Clinical Development, March, 2025
Intracranial AAV administration dose-dependently recruits B cells to inhibit the AAV redosing
【Other】
Gut, February, 2025
E-twenty-six-specific sequence variant 5 (ETV5) facilitates hepatocellular carcinoma progression and metastasis through enhancing polymorphonuclear myeloid-derived suppressor cell (PMN-MDSC)-mediated immunosuppression
【Other】
Cell Death & Disease, February, 2025
Mcm5 mutation leads to silencing of Stat1-bcl2 which accelerating apoptosis of immature T lymphocytes with DNA damage
【Other】
Molecular Therapy, February, 2025
Single-cell data-driven design of armed oncolytic virus to boost cooperative innate-adaptive immunity against cancer
【Other】
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