PiggyBac Transgenesis
Cyagen’s PiggyBac transgenesis platform enables efficient generation of stable, single-copy transgenic rodent models with consistent gene expression. It offers a reliable and cost-effective alternative for studying gene function and overexpression in vivo.
Flexible Design
Supports both plasmid and BAC-based constructs for diverse applications.
Consistent Expression
Ensures stable, reproducible transgene expression across tissues.
Single-Copy Integration
Minimizes silencing and variability by inserting one precise copy per site.
Technology
Applications
FAQs
How PiggyBac Transgenesis Works
PiggyBac transgenesis introduces a defined DNA fragment into the genome
via pronuclear injection. Unlike traditional methods, it integrates a
single copy of the transgene without vector backbone or sequence
truncation, resulting in more stable and predictable expression
patterns.
Workflow Highlights:
- Custom vector construction using PiggyBac elements
- Pronuclear injection of fertilized eggs
- Screening of founders via PCR-based genotyping
- Delivery of validated transgenic animals
PiggyBac-on-BAC: Enhanced Large Fragment Delivery
For researchers requiring large genomic regions with regulatory
elements, our PiggyBac-on-BAC method combines the capacity of BACs
(~100–300 kb) with the single-copy fidelity of PiggyBac. This enables
faithful gene expression patterns while avoiding multi-copy integration
artifacts commonly seen in conventional transgenics.
Citation Database
Molecular Therapy: Methods & Clinical Development, March, 2025
Intracranial AAV administration dose-dependently recruits B cells to inhibit the AAV redosing
【Other】
Gut, February, 2025
E-twenty-six-specific sequence variant 5 (ETV5) facilitates hepatocellular carcinoma progression and metastasis through enhancing polymorphonuclear myeloid-derived suppressor cell (PMN-MDSC)-mediated immunosuppression
【Other】
Cell Death & Disease, February, 2025
Mcm5 mutation leads to silencing of Stat1-bcl2 which accelerating apoptosis of immature T lymphocytes with DNA damage
【Other】
Molecular Therapy, February, 2025
Single-cell data-driven design of armed oncolytic virus to boost cooperative innate-adaptive immunity against cancer
【Other】
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