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PiggyBac Transgenesis
Cyagen’s PiggyBac transgenesis platform enables efficient generation of stable, single-copy transgenic rodent models with consistent gene expression. It offers a reliable and cost-effective alternative for studying gene function and overexpression in vivo.
Flexible Design
Supports both plasmid and BAC-based constructs for diverse applications.
Consistent Expression
Ensures stable, reproducible transgene expression across tissues.
Single-Copy Integration
Minimizes silencing and variability by inserting one precise copy per site.
Technology
Applications
FAQs
Technology
How PiggyBac Transgenesis Works
PiggyBac transgenesis introduces a defined DNA fragment into the genome via pronuclear injection. Unlike traditional methods, it integrates a single copy of the transgene without vector backbone or sequence truncation, resulting in more stable and predictable expression patterns.
Workflow Highlights:
  • Custom vector construction using PiggyBac elements
  • Pronuclear injection of fertilized eggs
  • Screening of founders via PCR-based genotyping
  • Delivery of validated transgenic animals
PiggyBac-on-BAC: Enhanced Large Fragment Delivery
For researchers requiring large genomic regions with regulatory elements, our PiggyBac-on-BAC method combines the capacity of BACs (~100–300 kb) with the single-copy fidelity of PiggyBac. This enables faithful gene expression patterns while avoiding multi-copy integration artifacts commonly seen in conventional transgenics.
Applications
Applications of PiggyBac Transgenesis
PiggyBac transgenesis is especially suited for in vivo studies that require stable, high-fidelity expression of transgenes. Its ability to support single-copy integration with minimal disruption makes it ideal for gene function analysis, regulatory element studies, therapeutic gene delivery, and modeling human diseases—including those involving large or complex sequences like lncRNAs. Whether using plasmid- or BAC-based constructs, PiggyBac offers consistent expression and experimental reproducibility across diverse research needs.
Explore Our Models & Services
Explore related transgenic technologies and services that complement your PiggyBac-based study design.
Transgenic Mice
Mice carrying foreign genes to explore gene overexpression and promoter activity.
Transgenic Rats
Rats engineered to express exogenous genes for disease and pathway research.
Regular Transgenesis
Pronuclear injection method for high-expression, multi-copy transgenes
BAC Transgenesis
Large-fragment delivery for accurate, native-like gene expression
Rodent Breeding
Scalable colony expansion with full genotyping support
MouseAtlas Model Library
Search and access curated genetically engineered mouse strains
FAQs
Frequently Asked Questions (FAQs)
What makes PiggyBac different from conventional pronuclear injection?
Unlike standard methods, PiggyBac inserts a single-copy transgene without vector backbone contamination.
What animal strains are supported?
We commonly use C57BL/6 for mice; custom strains and rats are available on request.
Can I integrate large genomic fragments with PiggyBac?
Yes—especially when using BAC constructs combined with PiggyBac in our PiggyBac-on-BAC strategy.
What is the average turnaround time?
Basic services typically take 8–10 weeks; guaranteed founder delivery may take up to 16 weeks.
Is this method suitable for therapeutic gene validation?
Yes. PiggyBac models are often used to overexpress therapeutic candidates in preclinical studies.
What Customers Say About Cyagen
Violet Shimmer
Stanford University
The service provided to us by Cyagen is now in press at Nature as an article.
Scarlett Rouge
Seattle Children’s Hospital
We are very pleased with the state-of-the-art professional transgenic services provided by Cyagen for our study published recently in Nature. We continue to use Cyagen’s transgenic services as it allows us to do better and more efficient research with transgenic mice.
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