PiggyBac Transgenesis
Cyagen’s PiggyBac transgenesis platform enables efficient generation of stable, single-copy transgenic rodent models with consistent gene expression. It offers a reliable and cost-effective alternative for studying gene function and overexpression in vivo.
Flexible Design
Supports both plasmid and BAC-based constructs for diverse applications.
Consistent Expression
Ensures stable, reproducible transgene expression across tissues.
Single-Copy Integration
Minimizes silencing and variability by inserting one precise copy per site.
Technology
Applications
FAQs
How PiggyBac Transgenesis Works
PiggyBac transgenesis introduces a defined DNA fragment into the genome
via pronuclear injection. Unlike traditional methods, it integrates a
single copy of the transgene without vector backbone or sequence
truncation, resulting in more stable and predictable expression
patterns.
Workflow Highlights:
- Custom vector construction using PiggyBac elements
- Pronuclear injection of fertilized eggs
- Screening of founders via PCR-based genotyping
- Delivery of validated transgenic animals
PiggyBac-on-BAC: Enhanced Large Fragment Delivery
For researchers requiring large genomic regions with regulatory
elements, our PiggyBac-on-BAC method combines the capacity of BACs
(~100–300 kb) with the single-copy fidelity of PiggyBac. This enables
faithful gene expression patterns while avoiding multi-copy integration
artifacts commonly seen in conventional transgenics.
What Customers Say About Cyagen
Violet Shimmer
Stanford University
The service provided to us by Cyagen is now in press at Nature as an article.
Scarlett Rouge
Seattle Children’s Hospital
We are very pleased with the state-of-the-art professional transgenic services provided by Cyagen for our study published recently in Nature. We continue to use Cyagen’s transgenic services as it allows us to do better and more efficient research with transgenic mice.
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