Diet-Induced Obesity (DIO) Mouse Model

DIO Mouse Model Description

After being fed a high-fat diet (60% fat content, D12492) for a twelve week period, C57BL/6N mice may be defined as Diet-Induced Obesity (DIO) mice. DIO mice exhibit phenotypes such as obesity, elevated fasting blood glucose, increased body fat accumulation, and insulin resistance. Cyagen offers DIO obese mouse models and provides one-stop drug efficacy and pharmacodynamic evaluation services based on the DIO mouse model.

Pharmacodynamic Evaluation Services for High-Fat Diet-Induced Obesity (DIO) Mouse Models

Case Validation:

Figure 1. Body Weight and Blood Glucose Testing in DIO Mice. Male mice at 6 weeks of age were fed a high-fat diet, and by the 25th week of induction, the average body weight of the mice was around 50g. The Normal Diet (ND) control group consisted of 10 mice; the High-Fat Diet (HFD) group consisted of 20 mice. Random blood glucose testing in the 25th week showed that the blood glucose levels in the HFD group were significantly higher than those in the control ND group.

Figure 2. Lipid Profile and Liver Function Indicators in DIO Mice. Blood biochemical data show no difference in triglycerides (TG) compared to the control group, while total cholesterol (T-CHO), HDL cholesterol (HDL-C), LDL cholesterol (LDL-C), aspartate aminotransferase (AS), and alanine aminotransferase (ALT) levels were significantly higher than those of the control group. ***p<0.001

Figure 3. Body composition in Lean and DIO mice. Body composition analysis was performed during diet treatments using a Conscious Small Animal Body Composition Analyzer to assess the fat mass and lean mass of each mouse (N=4).

Partner with Cyagen for Your DIO Model Research!

Cyagen’s Diet-Induced Obesity (DIO) Mouse Model offers a robust platform for studying obesity-related conditions and drug efficacy, showcasing key phenotypes such as elevated fasting blood glucose, increased body fat, and insulin resistance. Our model’s effectiveness is validated through significant findings in body weight, blood glucose levels, and lipid profiles, compared to normal diet controls. Not only do our models offer robust support for your research and development (R&D), but our comprehensive pharmacodynamic evaluation services can facilitate the preclinical studies of your model research.

Enhance your research outcomes with Cyagen’s DIO Mouse Models and one-stop preclinical evaluation services. Contact us today to learn how we can support your scientific endeavors and help accelerate your obesity research.