Type 2 Diabetes Mellitus (T2DM) Mouse Model

Diabetes Mellitus (DM) has become the third leading chronic disease among the population, following cancer and cardiovascular disease (CVD). This chronic disease is diagnosed when a person does not respond well to insulin or – as the disease progresses - cannot secrete sufficient insulin, resulting in increased glucose levels. Type 2 diabetes mellitus (T2DM) patients account for about 90% of all diabetic patients, and most patients with T2DM experience insulin resistance. The T2DM mouse model can be used in exploring the hypoglycemic effect of drugs on type 2 diabetic mice, which can be prepared by various methods - such as through diet, drugs, surgery, and gene editing.

1. Combination of high-fat diet and low-dose streptozotocin-treated mouse model

Construction Strategy: T2DM mouse model was constructed with a high-sugar and high-fat diet fed combined with low-dose intraperitoneal injection of streptozotocin (STZ).

Background: C57BL/6J

Weight: Greater than 18g, the average value is preferably about 20g.

Experimental groups: There are five experimental groups - model control group, positive control group, low-dose group, medium-dose group, and high-dose group.

Testing items: Body weight, food intake, glucose tolerance test (GTT), insulin tolerance test (ITT), fasting blood glucose test, random blood glucose test, serum biochemical test or kit method (ELISA test kit), physiological cage test (urine and feces collection), metabolic cage detection (indicators such as oxygen consumption, heat production, activity, etc.), tissue and organ collection and pathology analysis (H&E, Sirius red, Masson, Oil red, IHC, etc.), etc.

Type 2. Diet-induced obesity (DIO) Model

Principles: Continuous high-fat diet (HFD) induced obesity mouse, which can be used for obesity and type 2 diabetes (T2DM) research.

Background: C57BL/6J

Weight: Greater than 18g, the average value is preferably about 20g.

Experimental groups: There are five experimental groups - model control group, positive control group, low-dose group, medium-dose group, and high-dose group.

Test items: Body weight, food intake, glucose tolerance test (GTT), insulin tolerance test (ITT), fasting blood glucose test, random blood glucose test, serum biochemical test or kit method (ELISA test kit), physiological cage test (urine and feces collection), metabolic cage detection (indicators such as oxygen consumption, heat production, activity, etc.), tissue and organ collection and pathology analysis (H&E, Sirius red, Masson, Oil red, IHC, etc.), etc.

Type 2 Diabetes - Related genetically engineered mouse models