Ace2-KO Mice

Catalog Number: C001228

Strain Name: C57BL/6JCya-Ace2em4/Cya

Genetic Background: C57BL/6JCya

Reproduction: Homozygote x Homozygote

 

Strain Description

The protein encoded by the Angiotensin Converting Enzyme 2 (ACE2) gene belongs to the angiotensin converting enzyme family of dipeptidyl carboxydipeptidases and is homologous to Angiotensin I. ACE2 is expressed in a variety of human organs and has a strong affinity for Angiotensin I (AngⅠ) and Angiotensin II (AngⅡ) receptors. It catalyzes the cleavage of Ang I to angiotensin 1-9 (Ang1-9) and Ang II to angiotensin 1-7 (Ang1-7), which has a vasodilating and antihypertensive effect [1], and plays a role in the regulation of blood pressure, fluid balance, inflammation, cell proliferation, hypertrophy, and fibrosis, as well as in the regulation of cardiovascular and renal function and fertility [2]. ACE2 is also a common functional receptor for the spike protein of human coronavirus HCoV-NL63 and the severe acute respiratory syndrome coronaviruses SARS-CoV and SARS-CoV-2 [3].

This strain is a mouse Ace2 gene knockout model that uses gene editing technology to knock out the homologous gene of human ACE2 in mice. The knockout of the Ace2 gene will result in the absence of ACE2 protein expression, and this model can be used for the study of COVID-19. The homozygous Ace2-KO mice are viable and fertile.

Allele Type

The Ace2 gene is located on mouse chromosome X, and exon 3-18 of this gene was knocked out by gene targeting techniques.


Application

  • Research on Cardiovascular and Cardiac Contraction;
  • Research on Renal Function and Colitis;
  • Research on COVID-19 and SARS.

 


Publications

[1]Yu W, Ou X, Liu X, Zhang S, Gao X, Cheng H, Zhu B, Yan J. ACE2 contributes to the maintenance of mouse epithelial barrier function. Biochem Biophys Res Commun. 2020 Dec 17;533(4):1276-1282.
[2]Li JX, Xiao X, Teng F, Li HH. Myeloid ACE2 protects against septic hypotension and vascular dysfunction through Ang-(1-7)-Mas-mediated macrophage polarization. Redox Biol. 2024 Feb;69:103004.


References

[1]Donoghue M, Hsieh F, Baronas E, Godbout K, Gosselin M, Stagliano N, Donovan M, Woolf B, Robison K, Jeyaseelan R, Breitbart RE, Acton S. A novel angiotensin-converting enzyme-related carboxypeptidase (ACE2) converts angiotensin I to angiotensin 1-9. Circ Res. 2000 Sep 1;87(5):E1-9.
[2]Wang K, Gheblawi M, Oudit GY. Angiotensin Converting Enzyme 2: A Double-Edged Sword. Circulation. 2020 Aug 4;142(5):426-428.
[3]Zhang H, Rostami MR, Leopold PL, Mezey JG, O'Beirne SL, Strulovici-Barel Y, Crystal RG. Expression of the SARS-CoV-2 ACE2 Receptor in the Human Airway Epithelium. Am J Respir Crit Care Med. 2020 Jul 15;202(2):219-229.