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- ● Diet-Induced Obesity (DIO) Mouse Model
- ● Type 1 Diabetes Mellitus (T1DM) Mouse Model
- ● Type 2 Diabetes Mellitus (T2DM) Mouse Model
- ● Diet-induced Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) Mouse Model
- ● Chemically Induced Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) Mouse Model
- ● Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) Mouse Model (Gene-editing)
- ● Composite (Combined) Model - Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) Model
- ● Composite Model - Atherosclerosis Mouse Model
- ● Atherosclerosis Mouse Model (Gene-editing)
- ● Acute Pancreatitis Mouse Model
- ● Chronic Pancreatitis Mouse Model
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hACE2-All CDS-BALB/c Mice
Catalog Number: C001227
Strain Name: BALB/cAnCya-Ace2em1(hACE2)/Cya
Genetic Background: BALB/cAnCya
Reproduction: Heterozygous male x Homozygous female
Strain Description
Angiotensin-converting enzyme 2 (ACE2) is the predominant cell surface receptor for SARS-CoV-2 virus infection in humans, but due to interspecies differences, the SARS-CoV-2 virus is unable to bind to ACE2 receptors in most wild-type rodents, including mice. Replacement of mouse Ace2 with human ACE2 by gene editing techniques resulted in humanized ACE2 mice (hACE2) that stably express human ACE2 receptors for COVID-19 studies.
This strain is a mouse Ace2 gene humanization model that uses gene editing technology to replace the endogenous mouse Ace2 gene sequence with human ACE2 CDS, preserving the mouse signal peptide sequence and achieving hACE2 expression directed by the endogenous mouse Ace2 regulatory element. This gene is located on the mouse chromosome X and homozygous females and heterozygous males are viable and fertile.
Strain Strategy
Application
- COVID-19 and related diseases research.
- COVID-19 vaccine and antibody drug evaluation.
Validation Data
1. Detection of the expression of human ACE2 protein
Figure 1. Detection of the expression of human ACE2 protein in hACE2-All CDS-BALB/c mice. Different tissues from this model were taken and processed for immunohistochemical assays to detect the expression of human ACE2 (hACE2). The results showed that hACE2 protein was expressed in the intestine, lung, trachea, and kidney of this model.
Publications
[1]Chen J, Xu W, Li L, Yi L, Jiang Y, Hao P, Xu Z, Zou W, Li P, Gao Z, Tian M, Jin N, Ren L, Li C. Immunogenicity and protective potential of chimeric virus-like particles containing SARS-CoV-2 spike and H5N1 matrix 1 proteins. Front Cell Infect Microbiol. 2022 Jul 18;12:967493.
[2]Qu Q, Hao P, Xu W, Li L, Jiang Y, Xu Z, Chen J, Gao Z, Pang Z, Jin N, Li C. A Vaccine of SARS-CoV-2 S Protein RBD Induces Protective Immunity. Int J Mol Sci. 2022 Nov 8;23(22):13716.
