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Citations
IMMUNITY 47:107 (2017) IF=22.845
You may also be interested in
SD-Rosa26-LSL-tdTomato Rats
Catalog Number: CR003
Genetic Background: Crl:CD(SD)
Reproduction: Heterozygote x Heterozygote/Homozygote/WT or Homozygote x Homozygote
Strain Description
This strain is a conditional red fluorescent reporter rat strain carrying the expression cassette of the red fluorescent protein reporter gene (tdTomato). In the absence of Cre recombinase, the expression termination element (loxP-Stop-loxP, LSL) upstream of the tdTomato gene expression cassette blocks tdTomato expression. When this rat is crossed with a rat expressing Cre recombinase, Cre-positive cells, and their downstream differentiated lineages in the offspring undergo Cre recombinase-mediated deletion of the expression termination element between loxP sites, thereby achieving conditional expression of tdTomato protein. Strong tdTomato red fluorescent signals can then be observed in the relevant tissues or cells using a fluorescent microscope.
By mating SD-Rosa26-LSL-tdTomato rats with tissue-specific or inducible Cre rats, highly selective and controllable tdTomato protein expression time and location can be achieved. Validation data showed that strong red fluorescent signals were detected in the brain, heart, liver, spleen, kidney, thymus, pancreas, small intestine, large intestine, muscle, toes, ear, and tail of SD-Rosa26-LSL-tdTomato rats after Cre recombinase induction. Therefore, SD-Rosa26-LSL-tdTomato rats can be used for research in cell lineage tracing and specific gene expression regulation. Before the introduction of Cre recombinase, there may be deficient levels of tdTomato expression leakage in SD-Rosa26-LSL-tdTomato rats. Still, the tdTomato expression level mediated by Cre recombinase is significantly higher than the leakage level before induction.
Strain Strategy
The CAG promoter-loxP-PGK-Neo-6xSV40 pA-loxP-tdTomato-WPRE-BGH pA expression cassette was integrated into the Rosa26 safe harbor locus in rats through gene editing technology.
Application
- Cell lineage tracking;
- Cell division and differentiation studies;
- Gene expression regulation studies.
Validation Data
1. Expression of tdTomato protein in different tissues and organs of SD-Rosa26-LSL-tdTomato rats
Figure 1. Expression of red fluorescent protein (tdTomato) in different tissues and organs of SD-Rosa26-LSL-tdTomato rats. Tissues from the brain, heart, liver, spleen, kidney, thymus, pancreas, small intestine, large intestine, muscle, toes, ears, and tail of rats not induced by Cre recombinase (Cre-) and rats induced by Cre recombinase (Cre+) were taken separately. The occurrence of red fluorescence was observed under a fluorescence microscope. The results showed that there was no expression of tdTomato protein in the tissues of this strain before the induction of Cre recombinase, and the induction of Cre recombinase could lead to the appearance of red fluorescent signals of different intensities in almost all tissues.
Announcement
The ROSA26 locus where the LSL-tdTomato expression cassette is inserted is located on chromosome 4 of rats. Please avoid mating this strain of rats with Cre rats whose Cre recombinase gene expression cassette was integrated into the same chromosome as this strain.
