hF11 Mice

Product Number: C001272

Genetic Background: C57BL/6NCya

Reproduction: Homozygote x Homozygote

Strain Description

The F11 gene encodes coagulation factor XI of the blood coagulation cascade. This protein is present in plasma as a zymogen, which is a unique plasma coagulation enzyme because it exists as a homodimer consisting of two identical polypeptide chains linked by disulfide bonds. During activation of the plasma factor XI, an internal peptide bond is cleaved by factor XIIa (or XII) in each of the two chains, resulting in activated factor XIa, a serine protease composed of two heavy and two light chains held together by disulfide bonds. This activated plasma factor XI triggers the middle phase of the intrinsic pathway of blood coagulation by activating factor IX. Defects in this factor lead to Rosenthal syndrome, a blood coagulation abnormality.

This strain is a humanized model of F11 in which the CDS of human F11 (hF11) is expressed under the regulation of the mouse endogenous F11 gene promoter, and the expression of the mouse F11 gene is disrupted to reduce cross-reactivity. This model can be used for in vivo efficacy assessment and analysis of diseases associated with human F11, and homozygous hF11 mice are viable and fertile.

Strain Strategy

Figure 1. Diagram of the gene editing strategy for the generation of hF11 mice. The “human F11 CDS plus 3'UTR” gene expression element was inserted downstream of the mouse endogenous F11 gene promoter by gene editing technology.