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- ● Diet-Induced Obesity (DIO) Mouse Model
- ● Type 1 Diabetes Mellitus (T1DM) Mouse Model
- ● Type 2 Diabetes Mellitus (T2DM) Mouse Model
- ● Diet-induced Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) Mouse Model
- ● Chemically Induced Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) Mouse Model
- ● Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) Mouse Model (Gene-editing)
- ● Composite (Combined) Model - Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) Model
- ● Composite Model - Atherosclerosis Mouse Model
- ● Atherosclerosis Mouse Model (Gene-editing)
- ● Acute Pancreatitis Mouse Model
- ● Chronic Pancreatitis Mouse Model
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B6-Il2rg KO Mice
Catalog Number: C001374
Strain name: C57BL/6JCya-II2rgem1/Cya
Genetic Background: C57BL/6JCya
Reproduction: Homozygote x Homozygote
Strain Description
The interleukin-2 receptor subunit gamma (IL2Rg or CD132) gene encodes a protein that is an important signaling component of many interleukin receptors and is a common receptor subunit for several important immune factors, including IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21. IL2Rg is a glycoprotein expressed on the surface of most lymphocytes. In mammals, the IL2Rg gene is located on the X chromosome, and mutations in IL2Rg in humans can lead to X-linked severe combined immunodeficiency (X-SCID).
B6-Il2rg KO mice were obtained by knocking out the expression of the Il2rg gene in C57BL/6JCya mice, which are severely deficient in B and T cells in peripheral blood and bone marrow and partially deficient in the spleen, while the mice show a severe immunodeficient phenotype with almost complete absence of NK cells in peripheral blood, spleen, and bone marrow. This strain can be used for research in the fields of oncology, immunology, infectious disease, and stem cell biology.
Strain Strategy
Exon 2~6 of the Il2rg gene was deleted.
Strain Features
- Absence of NK cells;
- Severe deficiency of B and T cells in peripheral blood and bone marrow;
- Severe immunodeficiency.
Application
- Oncology: Research of tumor growth and metastasis and screening of anti-tumor drugs;
- Immunology: Research on the proliferation of immune cells and the mechanism of immune diseases;
- Infectious diseases: Research on the pathogenesis of viral and bacterial infectious diseases;
- Stem cell biology: Research on human-derived stem cell transplantation.
Validation Data
1. Detection of the T and B cell in the peripheral blood, spleen, and bone marrow
Figure 1. Detection of the T and B cell in the peripheral blood (PB), spleen, and bone marrow (BM) of B6-Il2rg KO and wild-type mice. Compared to wild-type mice, B6-Il2rg KO mice had severe defects in B and T cells in peripheral blood and bone marrow and partial reduction in the spleen.
2. Detection of the NK cell in the peripheral blood, spleen, and bone marrow
Figure 2. Detection of the NK cell in the peripheral blood (PB), spleen, and bone marrow (BM) of B6-Il2rg KO and wild-type mice. NK cells (CD3-CD335+) were almost completely absent in the peripheral blood, spleen, and bone marrow of B6-Il2rg KO mice compared to wild-type mice.
