Il12rb1 KO Mice

Catalog Number: C001214

Strain Name: C57BL/6NCya-Il12rb1^em1/Cya

Genetic Background: C57BL/6NCya

Reproduction: Homozygote x Homozygote

Strain Description

The Il12rb1 gene encodes a type I transmembrane protein that belongs to the hematopoietin receptor superfamily. This protein binds to interleukin-12 (IL-12) with low affinity and participates in IL-12 signal transduction. When combined with IL12RB2, it forms a functional high-affinity receptor for IL-12. The co-expression of this protein and IL12RB2 has been shown to create high-affinity IL-12 binding sites and reconstruct IL-12-dependent signal transduction. Mutations in the Il12rb1 gene impair the development of T lymphocytes that produce interleukin-17 and increase susceptibility to mycobacterial and Salmonella infections. Il12rb1 knockout (KO) homozygous mice exhibit reduced serum levels of interferon-gamma (IFN-γ) in response to recombinant IL-12 or lipopolysaccharide (LPS) treatment. Additionally, splenocytes from these mice activated by concanavalin A (ConA) in response to IL-12 cannot proliferate or secrete IFN-γ. These homozygous mice are viable and fertile.

Strain Strategy

The gene is located on chromosome 8, and exons 2 ~ 8 of the Il12rb1 gene have been knocked out.

Application

• Immune System-Related Research.

Publication
[1]Wang X, Jia L, Liu Y, Wang J, Qiu C, Li T, Zhang W, Zhu Z, Wu J, Wan Y. Immune Correlates of Disseminated BCG Infection in IL12RB1-Deficient Mice. Vaccines (Basel). 2022 Jul 19;10(7):1147.