What is the difference between Targeted Gene Editing and ES Cell Targeting?

Unlike ES cell targeting, which involves multiple rounds of cell screening and chimera breeding, Targeted Gene Editing allows direct modification at the zygote stage. This results in a faster timeline, reduced cost, and greater efficiency for generating custom mouse and rat models.

What types of genetic modifications are supported?

Our platform supports knockout, point mutation, conditional knockout, and large-fragment knockin (up to 15 kb). We also offer humanization and reporter knockin designs upon request.

How does Cyagen ensure specificity and minimize off-target effects?

We utilize our proprietary AI-powered sequence design engine, AlphaKnockout™, to identify the optimal genomic targeting guides (Guide Molecules) with minimal off-target potential. Each design undergoes rigorous in silico validation prior to embryo injection.

Can I use a specific mouse or rat strain for my model?

Yes. We routinely generate models on C57BL/6, FVB, SD, Wistar, and other commonly used strains. Additional strains can be accommodated upon request.

Is there a guarantee for model delivery?

Yes. Our Targeted Gene Editing services are backed by a 100% money-back guarantee if the promised genotype is not achieved.

Targeted Gene Editing for High-Precision Rodent Models

Targeted Gene Editing

Achieve precise genetic modifications in mice and rats with fast, cost-effective, and high-efficiency gene editing – powered by AI-optimized guide design and proprietary delivery techniques.

Wide Compatibility

Supports diverse model types across multiple strains and species.

High Editing Precision

AI-optimized guide molecules and enhanced HDR minimize off-target risks and improve editing fidelity.

Fast Turnaround

Accelerate your research with rapid model generation and efficient service delivery.

Technology

Applications

FAQs

Technology

How Targeted Gene Editing Works

Our Targeted Gene Editing platform introduces precise double-strand breaks (DSBs) at specific genomic locations using AI-optimized guide molecules. These breaks are repaired via homology-directed repair (HDR), enabling the accurate insertion, deletion, or replacement of genetic sequences.

Unlike traditional methods that primarily rely on non-homologous end joining (NHEJ)—which often leads to small insertions or deletions (indels)—our proprietary fertilization technique enhances HDR efficiency, making it ideal for complex model generation such as large-fragment knockin and conditional knockout.

Technology Workflow & Key Advantages

Feature	Conventional Gene Editing	Cyagen’s Targeted Gene Editing
Editing Stage	Zygote or ES cell (varies)	Direct embryo-stage editing
Targeting Strategy	Random DSB repair (often NHEJ)	HDR-optimized with proprietary fertilization
Precision	May result in unpredictable indels	High-precision insertion/deletion/mutation
Model Complexity	Limited for large edits	Supports up to 15 kb knockin / 400 kb knockout
Guide Molecule Design	Manual or vendor tools	AI-powered (via AlphaKnockout)
Efficiency	Variable	Significantly enhanced via HDR boosting
Applicable Species	Limited strains	Mouse (C57BL/6, FVB), Rat (SD, Wistar, others)

Looking for higher success rates and complex model capabilities?

Explore our TurboKnockout^® platform →

Applications

FAQs

Citation Database

Molecular Therapy: Methods & Clinical Development, March, 2025

Intracranial AAV administration dose-dependently recruits B cells to inhibit the AAV redosing

【Other】

Gut, February, 2025

E-twenty-six-specific sequence variant 5 (ETV5) facilitates hepatocellular carcinoma progression and metastasis through enhancing polymorphonuclear myeloid-derived suppressor cell (PMN-MDSC)-mediated immunosuppression

【Other】

Cell Death & Disease, February, 2025

Mcm5 mutation leads to silencing of Stat1-bcl2 which accelerating apoptosis of immature T lymphocytes with DNA damage

【Other】

Molecular Therapy, February, 2025

Single-cell data-driven design of armed oncolytic virus to boost cooperative innate-adaptive immunity against cancer

【Other】

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